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An optoelectronic ear oximeter (Hewlett-Packard, model 47201A) was evaluated as a noninvasive method for determining arterial oxygen saturation (SaO2) in human subjects during exposure to various levels of accelerative forces. This physiological calibration involved exposing five subjects, while breathing air and wearing the ear oximeter for 60 s to each of three levels of accelerative forces (3, 5, and 7 G); arterial blood samples were withdrawn concurrently. SaO2 was calculated indirectly from the oxygen tensions (PaO2) measured from the sampled arterial blood with a blood gas analyzer and corrected for pH and base excess. These data were compared, as were similar data taken from the same subjects breathing three different hypoxic gas mixtures while resitng at earth's gravity (1 G). Regression analyses of these data for both experimental groups (a, G exposure, or b, hypoxic exposure), comparing the ear-oximeter SaO2 with the calculated SaO2, showed the ear oximeter to be accurate with correlation coefficients of 0.95 and 0.98, respectively.