1. Structural characterization and comparative biomedical properties of phloroglucinol from Indian brown seaweeds
- Author
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Ramachandran Karthik, Pushpabai Rajaian Rajesh, Venkatesan Manigandan, Ravi Sheeba, and Ramachandran Saravanan
- Subjects
0106 biological sciences ,0301 basic medicine ,biology ,010604 marine biology & hydrobiology ,Conoides ,Phloroglucinol ,Plant Science ,Aquatic Science ,Secondary metabolite ,biology.organism_classification ,Mass spectrometry ,01 natural sciences ,Fluorescence spectroscopy ,03 medical and health sciences ,chemistry.chemical_compound ,Matrix-assisted laser desorption/ionization ,030104 developmental biology ,chemistry ,medicine ,Organic chemistry ,Chelation ,Fourier transform infrared spectroscopy ,Nuclear chemistry ,medicine.drug - Abstract
In the present study, phloroglucinol, a phenolic derivative, was extracted from the brown seaweeds Sargassum wightii, Sargassum tenerrimum and Turbinaria conoides. The secondary metabolite purified by reversed phase-high-performance liquid chromatography (RP-HPLC) was characterized using Fourier transform infrared spectroscopy (FT-IR), fluorescence spectroscopy, matrix-assisted laser desorption/ionization-time of flight/mass spectrometry (MALDI-TOF/MS) and H-1 nuclear magnetic resonance (NMR) spectroscopy. Phloroglucinol from S. tenerrimum exhibited higher total antioxidant (453.3 mg AscAE g(-1)), DPPH-scavenging (71.07 %), H2O2-scavenging (89.7 %) and metal chelating (14.28 %) activities. Anticoagulant activity was maximum in phloroglucinol from T. conoides (APTT 38.2 s, PT 22.7 s and FC 1.92 g dL(-1)). Phloroglucinol from S. wightii showed maximum lymphocyte migration inhibition (0.8 mm). Cytotoxicity and lactate dehydrogenase (LDH) leakage against MCF-7 cells was high in phloroglucinol from T. conoides (IC50 10.74 mu g mL(-1) and 56.17 %). These findings suggest that phloroglucinol from the selected brown seaweeds could be a rich functional food with appreciable biomedical properties.
- Published
- 2016