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Summary: The discharge of the toxic phenol‐polluted petro‐industrial effluents (PPPIE) has severe environmental negative impacts, thus it is mandatory to be treated before its discharge. The objective of this review was to discuss the sustainable application of microalgae in phenols degradation, with a special emphasis on the enzymes involved in this bioprocess and the factors affecting the success of PPPIE phycoremediation. Moreover, it confers the microalgae bioenergetic strategies to degrade different forms of phenols in PPPIE. It also points out the advantages of the latest application of bacteria, fungi and microalgae as microbial consortia in phenols biodegradation. Briefly, phycoremediation of PPPIE consumes carbon dioxide emitted from petro‐industries for; valorization of the polluted water to be reused and production of algal biomass which can act as a source of energy for such integrated bioprocess. Besides, the harvested algal biomass can feasibly produce; third‐generation biofuels, biorefineries, bioplastics, fish and animal feed, food supplements, natural dyes, antioxidants and many other valuable products. Consequently, this review precisely confirms that the phycoremediation of PPPIE is a win‐win process for a green environment and a sustainable future. Thus, to achieve the three pillars of sustainability; social, environmental and economic; it is recommendable to integrate PPPIE treatment with algal cultivation. This integrated process would overcome the problem of greenhouse gas emissions, global warming and climate change, solve the problem of water‐scarce, and protect the environment from the harmful negative impacts of PPPIE. [ABSTRACT FROM AUTHOR]

Aims: To conduct biological investigations and to evaluate the antimicrobial and antioxidant activities of the essential oils (EOs) extracted from Juniperus communis, J. scopulorum and J. horizontalis; to screen their mechanisms of action by conducting the cell membrane permeability assay (CMP); and to determine the possible cytotoxicity of the three EOs against human neuroblastoma cells (SH-SY5Y). Methods and Results: The antifungal activity was tested against four phytopathogenic fungi (Monilinia fructicola, Aspergillus niger, Penicillium expansum and Botrytis cinerea). The antibacterial activity was evaluated against two Gram-positive (G+ve) (Bacillus megaterium and Clavibacter michiganensis) and three Gram-negative (G--ve) bacterial strains (Pseudomonas fluorescens, P. syringae pv. phaseolicola and Xanthomonas campestris). Results showed that the three tested EOs have antifungal activity against M. fructicola and P. expansum and effective antibacterial activity against P. syringae pv. phaseolicola and B. megaterium. Moreover, the three EOs were evaluated for their ability to inhibit the growth of SH-SY5Y cells with MTT assay. J. communis EO was the more effective with an IC50 of 53•7 lg ml-1. The antioxidant capacity of the three EO did not differ as measured by the DPPH assay. Conclusions: The three tested juniper EOs showed promising antimicrobial and antioxidant activity and cytotoxic effects against human neuroblastoma cell line. Significance and Impact of the Study: The outfindings from this research showed promising antimicrobial effects of the three oils against the majority of the tested phytopathogens with a potential to utilize them as natural alternatives to synthetic drugs, the cause of global environmental problems, pathogen resistance and difficulty to control many post-harvest plant diseases. [ABSTRACT FROM AUTHOR]

Aims: This research was conducted to investigate the biocatalytic remediation of xenobiotics polluted seawater using two biocatalysts; whole bacterial cells of facultative aerobic halotolerant Corynebacterium variabilis Sh42 and its extracted crude enzymes. Methods and Results: One‐Factor‐at‐A‐Time technique and statistical analysis were applied to study the effect of initial substrate concentrations, pH, temperature, and initial biocatalyst concentrations on the batch biocatalytic degradation of three xenobiotic pollutants (2‐hydroxybiphenyl (2‐HBP), catechol and benzoic acid) in artificial seawater (salinity 3·1%). HPLC and gas‐chromatography mass spectroscopy analyses were utilized to illustrate the quantitative removal of the studied aromatic xenobiotic pollutants and their catabolic pathway. The results revealed that the microbial and enzymatic cultures followed substrate inhibition kinetics. Yano and Koga's equation showed the best fit for the biokinetic degradation rates of 2‐HBP and benzoic acid, whereas Haldane biokinetic model adequately expressed the specific biodegradation rate of catechol. The biokinetic results indicated the good efficiency and tolerance of crude enzyme for biocatalytic degradation of extremely high concentrations of aromatic pollutants than whole C. variabilis Sh42 cells. The monitored by‐products indicated that the catabolic degradation pathway followed an oxidation mechanism via a site‐specific monooxygenase enzyme. Benzoic acid and catechol were identified as major intermediates in the biodegradation pathway of 2‐HBP, which were then biodegraded through meta‐cleavage to 2‐hydroxymuconic semialdehyde. With time elapsed, the semialdehyde product was further biodegraded to acetaldehyde and pyruvic acid, which would be further metabolized via the bacterial TCA cycle. Conclusion: The batch enzymatic bioreactors performed superior‐specific biocatalytic degradation rates for all the studied xenobiotic pollutants. Significance and Impact of the Study: The enzymatic system of C. variabilis Sh42 is tolerable for toxic xenobiotics and different physicochemical environmental parameters. Thus, it can be recommended as an effective biocatalyst for biocatalytic remediation of xenobiotics polluted seawater. [ABSTRACT FROM AUTHOR]

Aims Clove extract has therapeutic potential as an antifungal drug, yet the mechanism of action remains ambiguous. Current study aimed to address the molecular process of the antifungal activity exerted by clove extract. Methods and Results The antifungal assay results showed that clove extract had some effects on all of the tested yeast. Propidium iodide staining assay showed cell membrane damage in Saccharomyces cerevisiae after treatment of clove extract for 30 h. Interestingly, SDS-polyacrylamide gel electrophoresis assays revealed that the protein expression of eukaryotic elongation factor 1 alpha ( eEF1A) was suppressed significantly after treatment with clove extract (not pure eugenol). Transcriptional analyses revealed that the TEF1 and TEF2 genes (translation elongation factor EF-1 alpha) encoding eEF1A were not disturbed with the addition of clove extract; however, the expression of related genes EFB1 (translation elongation factor 1 subunit beta), ENO2 (phosphopyruvate hydratase ENO2), GSP1 (Ran GTPase GSP1), RPP0 (ribosomal protein P0), YEF3 (translation elongation factor EF-3), TEF4 (translation elongation factor EF1B gamma), and RPS2 (ribosomal 40S subunit protein S2) increased significantly. Conclusions These results suggest that clove extract plays a role in disrupting growth and affecting yeast metabolism. eEF1A was affected by clove extract at the protein level but not at the transcriptional level. Significance and Impact of the Study This study is based on a detailed study of antifungal action exerted by clove extract, and proposed that down-regulation of eEF1A protein expression may contribute to its antifungal activity. These results may have clinical significance for future application of clove extract as a natural antifungal agent. [ABSTRACT FROM AUTHOR]