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Your search keyword '"Roberts, Marilyn C."' showing total 30 results
Start Over Author "Roberts, Marilyn C." Journal journal of antimicrobial chemotherapy
30 results on '"Roberts, Marilyn C."'

6. ResFinder 4.0 for predictions of phenotypes from genotypes

Author

Bortolaia, Valeria, primary, Kaas, Rolf S, additional, Ruppe, Etienne, additional, Roberts, Marilyn C, additional, Schwarz, Stefan, additional, Cattoir, Vincent, additional, Philippon, Alain, additional, Allesoe, Rosa L, additional, Rebelo, Ana Rita, additional, Florensa, Alfred Ferrer, additional, Fagelhauer, Linda, additional, Chakraborty, Trinad, additional, Neumann, Bernd, additional, Werner, Guido, additional, Bender, Jennifer K, additional, Stingl, Kerstin, additional, Nguyen, Minh, additional, Coppens, Jasmine, additional, Xavier, Basil Britto, additional, Malhotra-Kumar, Surbhi, additional, Westh, Henrik, additional, Pinholt, Mette, additional, Anjum, Muna F, additional, Duggett, Nicholas A, additional, Kempf, Isabelle, additional, Nykäsenoja, Suvi, additional, Olkkola, Satu, additional, Wieczorek, Kinga, additional, Amaro, Ana, additional, Clemente, Lurdes, additional, Mossong, Joël, additional, Losch, Serge, additional, Ragimbeau, Catherine, additional, Lund, Ole, additional, and Aarestrup, Frank M, additional

Published
2020
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30. CTX-M-15 extended-spectrum (beta)-lactamase from Nigerian Klebsiella pneumoniae.

Author

Soge OO, Queenan AM, Ojo KK, Adeniyi BA, and Roberts MC

Subjects
Anti-Bacterial Agents pharmacology, Base Sequence, Community-Acquired Infections epidemiology, DNA, Bacterial genetics, Drug Resistance, Multiple, Bacterial genetics, Escherichia coli genetics, Female, Humans, In Situ Hybridization, Isoelectric Focusing, Klebsiella Infections epidemiology, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae isolation & purification, Male, Microbial Sensitivity Tests, Molecular Sequence Data, Nigeria epidemiology, Plasmids, Urinary Tract Infections epidemiology, Community-Acquired Infections microbiology, Genes, Bacterial, Klebsiella Infections microbiology, Klebsiella pneumoniae enzymology, Urinary Tract Infections microbiology, beta-Lactamases genetics
Abstract

Objectives: In this study, extended-spectrum beta-lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria., Methods: The beta-lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the bla(CTX-M) genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNA-DNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants., Results: All 30 isolates produced at least one beta-lactamase. Seventeen of the isolates were resistant to cefotaxime, and had > or =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have bla(CTX-M) genes that were associated with large plasmids (> or =58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 beta-lactamases. The ISEcp1 element was located upstream of bla(CTX-M-15) in the same position as previously described. In addition, 23 of the isolates produced TEM beta-lactamases, 27 produced SHV beta-lactamases and four produced AmpC beta-lactamases., Conclusions: Thirty K. pneumoniae produced multiple beta-lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa.

Published
2006
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