1. Inducible colistin resistance via a disrupted plasmid-borne mcr-1 gene in a 2008 Vietnamese Shigella sonnei isolate.
- Author
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Pham Thanh D, Thanh Tuyen H, Nguyen Thi Nguyen T, Chung The H, Wick RR, Thwaites GE, Baker S, and Holt KE
- Subjects
- Conjugation, Genetic, Gene Transfer, Horizontal, Genome, Bacterial, Humans, Polymerase Chain Reaction, Sequence Analysis, DNA, Shigella sonnei genetics, Shigella sonnei isolation & purification, Transcriptional Activation, Vietnam, Anti-Bacterial Agents pharmacology, Colistin pharmacology, Drug Resistance, Bacterial, Gene Expression Regulation, Bacterial, Genes, Bacterial, Plasmids, Shigella sonnei drug effects
- Abstract
Objectives: The objective of this study was to assess the presence of mcr-1 in Shigella sonnei isolated in Vietnam., Methods: WGS data were analysed for the presence of the mcr-1 gene sequence. The association of mcr-1 with a plasmid was assessed by PCR and by conjugation., Results: Through genome sequencing we identified a plasmid-associated inactive form of mcr-1 in a 2008 Vietnamese isolate of Shigella sonnei. The plasmid was conjugated into Escherichia coli and mcr-1 was activated upon exposure to colistin, resulting in highly colistin-resistant transconjugants., Conclusions: This is the first description of the mcr-1 gene in Shigella, which is atypical given that colistin is not ordinarily used to treat diarrhoea. Our data suggest the mcr-1 gene has been circulating in human-restricted pathogens for some time but likely carries a selective fitness cost., (© The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2016
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