1. Dissemination of carbapenemase-producing Enterobacteriaceae in France, 2012
- Author
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Patrice Nordmann, Laurent Dortet, and Gaelle Cuzon
- Subjects
DNA, Bacterial ,Microbiology (medical) ,Carbapenemase-Producing Enterobacteriaceae ,Imipenem ,Carbapenem ,medicine.drug_class ,Antibiotics ,Biology ,Polymerase Chain Reaction ,beta-Lactam Resistance ,beta-Lactamases ,law.invention ,Microbiology ,Antibiotic resistance ,Bacterial Proteins ,Enterobacteriaceae ,law ,polycyclic compounds ,medicine ,Humans ,Pharmacology (medical) ,Polymerase chain reaction ,Pharmacology ,Enterobacteriaceae Infections ,K pneumoniae ,Sequence Analysis, DNA ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Anti-Bacterial Agents ,Infectious Diseases ,Carbapenems ,France ,medicine.drug - Abstract
Objectives Carbapenem-resistant Enterobacteriaceae isolates (n = 1485) were received at the French Associated National Reference Center for Antibiotic Resistance in 2012 and were characterized for their mechanism of resistance to carbapenems. Methods Carbapenemase production was detected using the biochemical-based Carba NP test, based on the detection of in vitro hydrolysis of imipenem. All isolates with a positive Carba NP test result were characterized by PCR and sequencing. Results Carbapenemase production was identified in 23.1% of the isolates. The main carbapenemase type identified was OXA-48 and derivatives (75.5%). An overseas source was clearly demonstrated for only 27.6% of the isolates. Conclusions OXA-48 and derivatives are now the most prevalent carbapenemases in France, with a possible spread of these producers in the community.
- Published
- 2013
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