6 results on '"Phosphoric Diester Hydrolases immunology"'
Search Results
2. Analysis of basophil activation in patients with aspirin-exacerbated respiratory disease.
- Author
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Mitsui C, Kajiwara K, Ono E, Watai K, Hayashi H, Kamide Y, Fukutomi Y, Sekiya K, Tsuburai T, Yamamoto K, and Taniguchi M
- Subjects
- Adult, Aged, Asthma chemically induced, Female, Humans, Male, Middle Aged, Phosphoric Diester Hydrolases immunology, Pyrophosphatases immunology, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Aspirin adverse effects, Asthma immunology, Basophils immunology
- Published
- 2017
- Full Text
- View/download PDF
3. Clinical relevance of the Hevea brasiliensis lipid transfer protein Hev b 12.
- Author
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Faber MA, Sabato V, Bridts CH, Nayak A, Beezhold DH, and Ebo DG
- Subjects
- Adult, Antigens, Plant chemistry, Antigens, Plant pharmacology, Basophils drug effects, Basophils pathology, Cells, Cultured, Child, Female, Gene Expression, Hevea chemistry, Humans, Immunoglobulin E blood, Latex Hypersensitivity genetics, Latex Hypersensitivity pathology, Male, Middle Aged, Phosphoric Diester Hydrolases genetics, Phosphoric Diester Hydrolases immunology, Plant Proteins chemistry, Plant Proteins pharmacology, Pyrophosphatases genetics, Pyrophosphatases immunology, Skin Tests, Tetraspanin 30 genetics, Tetraspanin 30 immunology, Antigens, Plant immunology, Basophils immunology, Hevea immunology, Latex Hypersensitivity diagnosis, Latex Hypersensitivity immunology, Plant Proteins immunology
- Published
- 2015
- Full Text
- View/download PDF
4. The safety and efficacy of sublingual and oral immunotherapy for milk allergy.
- Author
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Keet CA, Frischmeyer-Guerrerio PA, Thyagarajan A, Schroeder JT, Hamilton RG, Boden S, Steele P, Driggers S, Burks AW, and Wood RA
- Subjects
- Administration, Oral, Administration, Sublingual, Adolescent, Basophils immunology, Child, Double-Blind Method, Female, Histamine immunology, Humans, Immunoglobulin E blood, Immunoglobulin G blood, Intracellular Signaling Peptides and Proteins immunology, Male, Milk Hypersensitivity blood, Milk Hypersensitivity immunology, Phosphoric Diester Hydrolases immunology, Protein-Tyrosine Kinases immunology, Pyrophosphatases immunology, Remission Induction, Skin Tests, Syk Kinase, Tetraspanin 30 immunology, Desensitization, Immunologic, Milk Hypersensitivity therapy, Milk Proteins administration & dosage
- Abstract
Background: Oral immunotherapy (OIT) and sublingual immunotherapy (SLIT) are potential therapies for food allergy, but the optimal method of administration, mechanism of action, and duration of response remain unknown., Objective: We sought to explore the safety and efficacy of OIT and SLIT for the treatment of cow's milk (CM) allergy., Methods: We randomized children with CM allergy to SLIT alone or SLIT followed by OIT. After screening double-blind, placebo-controlled food challenges and initial SLIT escalation, subjects either continued SLIT escalation to 7 mg daily or began OIT to either 1000 mg (the OITB group) or 2000 mg (the OITA group) of milk protein. They were challenged with 8 g of milk protein after 12 and 60 weeks of maintenance. If they passed the 60-week challenge, therapy was withdrawn, with challenges repeated 1 and 6 weeks later. Mechanistic correlates included end point titration skin prick testing and measurement of CM-specific IgE and IgG(4) levels, basophil histamine release, constitutive CD63 expression, CD203c expression, and intracellular spleen tyrosine kinase levels., Results: Thirty subjects with CM allergy aged 6 to 17 years were enrolled. After therapy, 1 of 10 subjects in the SLIT group, 6 of 10 subjects in the SLIT/OITB group, and 8 of 10 subjects in the OITA group passed the 8-g challenge (P = .002, SLIT vs OIT). After avoidance, 6 of 15 subjects (3 of 6 subjects in the OITB group and 3 of 8 subjects in the OITA group) regained reactivity, 2 after only 1 week. Although the overall reaction rate was similar, systemic reactions were more common during OIT than during SLIT. By the end of therapy, titrated CM skin prick test results and CD63 and CD203c expression decreased and CM-specific IgG(4) levels increased in all groups, whereas CM-specific IgE and spontaneous histamine release values decreased in only the OIT group., Conclusion: OIT was more efficacious for desensitization to CM than SLIT alone but was accompanied by more systemic side effects. Clinical desensitization was lost in some cases within 1 week off therapy., (Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
5. CD203c expression on human basophils is associated with asthma exacerbation.
- Author
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Ono E, Taniguchi M, Higashi N, Mita H, Kajiwara K, Yamaguchi H, Tatsuno S, Fukutomi Y, Tanimoto H, Sekiya K, Oshikata C, Tsuburai T, Tsurikisawa N, Otomo M, Maeda Y, Hasegawa M, Miyazaki E, Kumamoto T, and Akiyama K
- Subjects
- Adult, Aged, Antibodies, Anti-Idiotypic immunology, Antigens, CD biosynthesis, Antigens, CD immunology, Antigens, Dermatophagoides immunology, Antigens, Differentiation, T-Lymphocyte biosynthesis, Antigens, Differentiation, T-Lymphocyte immunology, Arthropod Proteins, Asthma immunology, Basophils immunology, Cell Separation, Cysteine Endopeptidases, Female, Flow Cytometry, Histamine Release immunology, Humans, Interleukin-3 immunology, Interleukin-3 metabolism, Lectins, C-Type biosynthesis, Lectins, C-Type immunology, Male, Middle Aged, Phosphoric Diester Hydrolases immunology, Platelet Membrane Glycoproteins biosynthesis, Platelet Membrane Glycoproteins immunology, Pyrophosphatases immunology, Respiratory Function Tests, Tetraspanin 30, Young Adult, Asthma metabolism, Basophils metabolism, Biomarkers analysis, Phosphoric Diester Hydrolases biosynthesis, Pyrophosphatases biosynthesis
- Abstract
Background: CD203c is a basophil cell surface marker used to diagnose and monitor various allergic diseases, but its relationship to asthma is not clear., Objective: We determined whether CD203c expression levels are associated with stable and exacerbated asthma., Methods: We used flow cytometry to compare spontaneous expression levels of surface markers on basophils from patients with stable or exacerbated asthma and from healthy subjects. Longitudinal changes in these expression levels were measured after basophil stimulation by IgE-dependent or IgE-independent mechanisms and compared with patients' asthma status., Results: Spontaneous expression levels of CD203c were significantly higher on basophils from patients with asthma exacerbation than patients with stable asthma or healthy subjects. In contrast, no differences in spontaneous expression levels of CD63 or CD69 were observed among the 3 groups. Anti-IgE-induced expression of CD203c significantly increased in basophils during asthma exacerbation (P = .005). Low concentrations of Dermatophagoides pteronyssinus or IL-3 induced higher expression levels of CD203c during asthma exacerbation than during clinical improvement; induction of CD203c expression by these antigens therefore correlates with asthma control. In the patients with clinical improvement, there was a correlation between spontaneous CD203c expression levels and the percent predicted values of FEV(1) (r = -0.761; P = .022)., Conclusion: Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission. Basophil expression levels of CD203c might therefore be used to monitor asthma in patients., (Copyright 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
6. Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients.
- Author
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Falcone FH, Telford G, Hooi D, Brown AP, Seabra R, Feary J, Venn A, Britton J, and Pritchard DI
- Subjects
- Animals, Antibodies, Helminth blood, Antigens, CD immunology, Antigens, CD metabolism, Antigens, Helminth immunology, Basophils parasitology, Double-Blind Method, Humans, Immunoglobulin E blood, Immunoglobulin G blood, Immunoglobulin M blood, Necatoriasis parasitology, Phosphoric Diester Hydrolases immunology, Phosphoric Diester Hydrolases metabolism, Platelet Membrane Glycoproteins immunology, Platelet Membrane Glycoproteins metabolism, Pyrophosphatases immunology, Pyrophosphatases metabolism, Tetraspanin 30, Basophils immunology, Necator americanus immunology, Necatoriasis immunology
- Abstract
Background: Parasitic worms induce a strong, polarized T(H)2-type immune response. The kinetics of gastrointestinal nematode-induced T(H)2-type responses, especially in the context of primary infection, have been extensively studied in experimental infection models but not in human subjects., Objective: We sought to determine the kinetics of basophil sensitization in subjects infected with Necator americanus during the first 12 weeks after infection., Methods: Thirty nonasthmatic subjects with allergic rhinoconjunctivitis were randomized in a double-blind manner to cutaneous administration of either 10 hookworm infective larvae or histamine placebo. Blood samples were taken at regular intervals for 12 weeks, and basophil activation was determined in whole blood by measuring CD63 and CD203c levels on stimulation with N americanus excretions/secretions. Parasite-specific immunoglobulin responses were assessed by means of ELISA and Western blotting., Results: Median values reflecting basophil activation (CD203c/CD63 double-positive cells) in the excretion/secretion-stimulated infected group steadily increased after week 4, consistently achieving statistical significance compared with the placebo group between 6 and 12 weeks after infection. Only parasite-specific IgM levels increased significantly during this period, whereas total and parasite-specific IgE levels did not differ between groups., Conclusion: Basophils are sensitized early in the context of a low-dose primary infection with N americanus in the absence of measurable total and specific IgE serum level increase.
- Published
- 2009
- Full Text
- View/download PDF
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