Search

Your search keyword '"Delivanis DA"' showing total 2 results
Start Over Author "Delivanis DA" Journal journal of allergy and clinical immunology
2 results on '"Delivanis DA"'

1. Increased serum CRH levels with decreased skin CRHR-1 gene expression in psoriasis and atopic dermatitis.

Author

Vasiadi M, Therianou A, Sideri K, Smyrnioti M, Sismanopoulos N, Delivanis DA, Asadi S, Katsarou-Katsari A, Petrakopoulou T, Theoharides A, Antoniou C, Papadavid E, Stavrianeas N, Kalogeromitros D, and Theoharides TC

Subjects
Adult, Aged, Dermatitis, Atopic genetics, Disease Progression, Female, Genetic Association Studies, Humans, Inflammation Mediators metabolism, Interleukin-8 blood, Male, Mast Cells immunology, Mast Cells pathology, Middle Aged, Psoriasis genetics, Skin pathology, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Young Adult, Corticotropin-Releasing Hormone blood, Dermatitis, Atopic immunology, Gene Expression Regulation, Psoriasis immunology, Receptors, Corticotropin-Releasing Hormone genetics
Published
2012
Full Text
View/download PDF

2. Human mast cell degranulation and preformed TNF secretion require mitochondrial translocation to exocytosis sites: relevance to atopic dermatitis.

Author

Zhang B, Alysandratos KD, Angelidou A, Asadi S, Sismanopoulos N, Delivanis DA, Weng Z, Miniati A, Vasiadi M, Katsarou-Katsari A, Miao B, Leeman SE, Kalogeromitros D, and Theoharides TC

Subjects
Adolescent, Adult, Antigens administration & dosage, Biological Transport, Active, Calcineurin genetics, Calcineurin metabolism, Calcium metabolism, Case-Control Studies, Cell Degranulation drug effects, Cell Degranulation immunology, Cells, Cultured, Child, Dermatitis, Atopic genetics, Dermatitis, Atopic immunology, Dynamins, Exocytosis physiology, Female, GTP Phosphohydrolases antagonists & inhibitors, GTP Phosphohydrolases genetics, GTP Phosphohydrolases metabolism, Humans, Immunoglobulin E administration & dosage, Male, Mast Cells drug effects, Mast Cells immunology, Mast Cells ultrastructure, Microscopy, Electron, Transmission, Microtubule-Associated Proteins antagonists & inhibitors, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Middle Aged, Mitochondria physiology, Mitochondrial Proteins antagonists & inhibitors, Mitochondrial Proteins genetics, Mitochondrial Proteins metabolism, RNA, Small Interfering genetics, Substance P administration & dosage, Substance P genetics, Young Adult, Cell Degranulation physiology, Dermatitis, Atopic physiopathology, Mast Cells physiology, Tumor Necrosis Factor-alpha physiology
Abstract

Background: Mast cells derive from hematopoietic cell precursors and participate in tissue allergic, immune, and inflammatory processes. They secrete many mediators, including preformed TNF, in response to allergic, neuropeptide, and environmental triggers. However, regulation of mast cell degranulation is not well understood., Objective: We investigated the role of mitochondrial dynamics in degranulation of human cultured mast cells., Methods: Human umbilical cord blood-derived mast cells (hCBMCs) and Laboratory of Allergic Diseases 2 (LAD2) mast cells were examined by confocal and differential interference contrast microscopy during activation by IgE/antigen and substance P (SP). Mast cells in control and atopic dermatitis (AD) skin were evaluated by transmission electron microscopy. LAD2 cells were pretreated with mitochondrial division inhibitor, a dynamin-related protein 1 (Drp1) inhibitor, and small interfering RNA for Drp1, which is necessary for mitochondrial fission and translocation. Calcineurin and Drp1 gene expression was analyzed in stimulated LAD2 cells and AD skin biopsies., Results: Stimulation of hCBMCs with IgE/antigen or LAD2 cells with SP leads to rapid (30 minutes) secretion of preformed TNF. Degranulation is accompanied by mitochondrial translocation from a perinuclear location to exocytosis sites. Extracellular calcium depletion prevents these effects, indicating calcium requirement. The calcium-dependent calcineurin and Drp1 are activated 30 minutes after SP stimulation. Reduction of Drp1 activity by mitochondrial division inhibitor and decrease of Drp1 expression using small interfering RNA inhibit mitochondrial translocation, degranulation, and TNF secretion. Mitochondrial translocation is also evident by transmission electron microscopy in skin mast cells from AD biopsies, in which gene expression of calcineurin, Drp1, and SP is higher than in normal skin., Conclusion: Human mast cell degranulation requires mitochondrial dynamics, also implicated in AD., (Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results