1. Glufosinate Resistance Level is Proportional to Phosphinothricin Acetyltransferase Gene Expression in Glufosinate-Resistant Maize
- Author
-
Alfredo Junior Paiola Albrecht, Fábio Henrique Krenchinski, Caio Antonio Carbonari, Mariana de Lara Campos Arcuri, Ivan de Godoy Maia, Edivaldo Domingues Velini, and V.J.S. Cesco
- Subjects
0106 biological sciences ,animal structures ,Pesticide resistance ,animal diseases ,Biology ,01 natural sciences ,Marker gene ,Zea mays ,chemistry.chemical_compound ,fluids and secretions ,Acetyltransferases ,Gene Expression Regulation, Plant ,Gene expression ,Ammonium Compounds ,Herbicide resistance ,Gene ,Plant Proteins ,Herbicides ,Aminobutyrates ,04 agricultural and veterinary sciences ,General Chemistry ,Molecular biology ,body regions ,Plant Leaves ,Glufosinate ,chemistry ,Genetic marker ,cardiovascular system ,040103 agronomy & agriculture ,Phosphinothricin acetyltransferase ,0401 agriculture, forestry, and fisheries ,General Agricultural and Biological Sciences ,010606 plant biology & botany ,Herbicide Resistance - Abstract
Phosphinothricin acetyltransferase ( pat) gene confers resistance to glufosinate by transforming this herbicide into N-acetyl-l-glufosinate (NAG). The pat gene was inserted in six maize hybrids (Herculex, Agrisure TL, Herculex Yieldgard, Leptra, Viptera 3, Power Core) as a selectable marker, and its expression was evaluated by qPCR in comparison with the maize glufosinate-susceptible cultivar VTPRO. In addition, the levels of NAG, glufosinate degradation, ammonia accumulation, electron transport rate (ETR), visual injury, and biomass were also investigated. The VTPRO, Herculex, Agrisure, and Viptera showed lower pat gene expression, and consequently lower NAG contents and glufosinate degradation, as well as reduced ETR and biomass accumulation. In contrast, greater ammonia accumulation and higher visual injury were observed. The ranking of pat gene expression was LeptraPower CoreHerculex Yieldgard ≫ HerculexAgrisure TL = Viptera 3VTPRO. This gene expression was proportional to the glufosinate resistance level observed in each maize hybrid.
- Published
- 2018