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The aim of this research was to determine the total phenolic content (TPC), antioxidation, antiaging, and antibacterial activities of Carissa carandas Linn., and aims at the novel plant sources which is utilized for their cosmeceutical applications. The two conditions (fresh and dried) and three stages (unripe, ripe, and fully ripe) of C. carandas were extracted by ethanolic maceration. Folin-Ciocalteu assay was used for determining the TPC. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used for estimating antioxidant activity. The inhibitory tyrosinase activities were measured using the modified dopachrome assay. Antiaging was evaluated by inhibition of collagenase and elastase, and antibacterial activities. The result of six extracts from C. carandas showed that the highest phenolic content and elastase inhibition of the fresh fruit in fully ripe stage were 100.31 ± 2.64 mg GAE/g extract and 14.11% ± 0.95%, respectively. The fresh fruit in the unripe stage showed that the strongest percentage of DPPH IC 50 and collagenase inhibitory activity were 29.11 ± 0.23 μg/mL and 85.94% ± 2.21%, respectively. The ethanolic extract of unripe dried fruit exhibited the highest antioxidant activity in the of ABTS assay, with an IC 50 of 0.17 ± 0.01 μg/mL. The MBC displayed the dried fruit ripe stage anti Cutibacterium acnes, Staphylococcus epidermidis , and Staphylococcus aureus strains were 25.0, 25.0, and 16.25 mg/mL, respectively. The fresh fruit in the ripe stage showed that the strongest inhibition tyrosinase was 93.88% ± 5.64%. The conclusion of this research indicates that the fresh fruit of C. carandas fruit extracts has high potential as a novel cosmeceuticals' applications to antiaging and skin whitening. The dried fruit in ripe stage extract has the most effective ingredient for antiacne products., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of Advanced Pharmaceutical Technology & Research.)

Centella asiatica , Nelumbo nucifera Gaertn , and Hibiscus sabdariffa have been used as medicinal plants in Thailand. They are sources of phytochemicals that applications for esthetic and healthcare. The aim of this research was to examine the phytochemical constituents and anti-aging potential of these plants. The phytochemical compounds were performed using gas chromatography-mass spectrometry. The anti-aging activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sunfonic acid) (ABTS), anti-collagenase, and anti-elastase assays. The main interest phytochemical compounds of ethanolic extracts of C. asiatica , N. nucifera , H. sabdariffa were ethanol, 2-(-Octadecenyloxy), γ-sitosterol and hexadecanoic acid, and ethyl ester, respectively. The DPPH half-maximal inhibitory concentration (IC 50 ) results of C. asiatica , N. nucifera, and H. sabdariffa were 0.32 ± 0.01, 0.34 ± 0.00, and 0.35 ± 0.01 mg/mL, respectively. The ABTS result of H. sabdariffa extract showed high inhibitory activity at IC 50 of the extract was 0.62 ± 0.12 mg/mL. The percentage of collagenase inhibition of C. asiatica , N. nucifera , and H. sabdariffa at 1.0 mg/mL was 78.13 ± 4.42, 85.94 ± 2.21, and 90.63 ± 0.00, respectively. The C. asiatica extract had a high percentage of elastase inhibition. Consequently, these research results suggest that phytochemicals may also provide a range of esthetic and health benefits. The phytochemical constituent could be used as anti-aging active ingredient for cosmetic and pharmaceutical industrials., Competing Interests: There are no conflicts of interest., (Copyright: © 2020 Journal of Advanced Pharmaceutical Technology & Research.)