1. HIV and SIV Envelope Glycoproteins Induce Phospholipase A2 Activation in Human and Macaque Lymphocytes
- Author
-
Philippe Yaba, Hahn T. Nguyen, Marie-Claude Georges-Courbot, Alain J. Georges, Virginie Poaty-Mavoungou, André Délicat, Elie Mavoungou, and Françoise Russo-Marie
- Subjects
Boron Compounds ,CD4-Positive T-Lymphocytes ,Male ,viruses ,Immunology ,HIV Envelope Protein gp120 ,Lymphocyte Activation ,medicine.disease_cause ,Macaque ,Phospholipases A ,Virus ,Phospholipase A2 ,Viral envelope ,Virology ,biology.animal ,medicine ,Animals ,Humans ,Immunology and Allergy ,Fluorescent Dyes ,chemistry.chemical_classification ,Arachidonic Acid ,Cell fusion ,biology ,env Gene Products, Human Immunodeficiency Virus ,Gene Products, env ,virus diseases ,Simian immunodeficiency virus ,Flow Cytometry ,biology.organism_classification ,Enzyme Activation ,Macaca fascicularis ,Phospholipases A2 ,chemistry ,HIV-2 ,Liposomes ,Lentivirus ,HIV-1 ,Phosphatidylcholines ,biology.protein ,Female ,Simian Immunodeficiency Virus ,lipids (amino acids, peptides, and proteins) ,Glycoprotein - Abstract
We investigated the early interactions between HIV-1, HIV-2, and simian immunodeficiency virus (SIV) envelope glycoproteins gp120(IIIB), gp105(ROD), and gp120(mac251), and human and macaque cells of the lymphocytic series. Our results demonstrate that the soluble viral glycoproteins induce a specific phospholipase A2 (PLA2) activation in lymphocytes through CD4. This PLA2 activation was induced after envelope glycoprotein-CD4 interaction and, because of its local membrane-destabilizing effect, may have important implications for preparing the lymphocyte membrane for fusion with the viral particle. However, this effect is not sufficient to accomplish fusion. These data indicate that the specific step of fusion may be downstream from PLA2 activation.
- Published
- 1997
- Full Text
- View/download PDF