1. Cbfa1 expression in vascular smooth muscle cells may be elevated by increased nitric oxide/iNOS
- Author
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Margaret Gori Mouro, Simone Geraldini, Aluizio B. Carvalho, Maria Aparecida da Glória, and Elisa Mieko Suemitsu Higa
- Subjects
Lipopolysaccharides ,Male ,medicine.medical_specialty ,Vascular smooth muscle ,Fatores de Ligação ao Core ,020205 medical informatics ,Lipopolysaccharide ,Myocytes, Smooth Muscle ,Core Binding Factor Alpha 1 Subunit ,02 engineering and technology ,lcsh:RC870-923 ,Nitric Oxide ,Muscle, Smooth, Vascular ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Renal Artery ,Internal medicine ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,Animals ,Humans ,Rats, Wistar ,0303 health sciences ,Kidney diseases ,biology ,Acridine orange ,Core Binding Factors ,Osteoblast ,General Medicine ,lcsh:Diseases of the genitourinary system. Urology ,Rats ,Óxido Nítrico Sintase ,Nitric oxide synthase ,CTL ,Endocrinology ,medicine.anatomical_structure ,030301 anatomy & morphology ,chemistry ,Nefropatias ,biology.protein ,Immunohistochemistry ,lipids (amino acids, peptides, and proteins) ,Original Article ,Miócitos de Músculo Liso ,Nitric Oxide Synthase - Abstract
Introduction: Vascular calcification is a common complication of chronic kidney disease. Osteoblast differentiation factor (Cbfa1) is present in histologic sections of arteries from patients with end-stage renal disease. Vascular smooth muscle cells (VSMC) can dedifferentiate to osteoblast-like cells, possibly by up-regulation of Cbfa1. There is evidence that the production of nitric oxide (NO) may have an important role in the regulation of osteoblast metabolism. The aim of this study is to evaluate whether increased NO/iNOS expression causes an increase in cbfa1 expression in VSMC. Methods: VSMC were obtained from renal artery of Wistar male rats, treated for 72 hours with lipopolysaccharide (LPS), ß-glycerophosphate (BGF), a donor of phosphate and aminoguanidine (AG), an inhibitor of iNOS, in the following groups: CTL (control), LPS, BGF, LPS + BGF, and LPS + AG. NO synthesis was determined by chemiluminescence. Cbfa1 and iNOS mRNA expressions were analyzed by RT-PCR, Cbfa1 protein expression by immunohistochemistry and cellular viability by acridine orange. Results: Cbfa1 and iNOS mRNA expressions were higher in LPS and LPS+ BGF vs CTL (p < 0.05), and they were lower in LPS+AG vs LPS (p < 0.05). The Cbfa1 in the groups LPS and LPS+BGF also resulted in a higher value compared to CTL (p < 0.05), and in LPS+AG it was lower compared to LPS (p < 0.05). NO was higher in LPS and LPS+BGF compared to CTL group (p < 0.05) and lower in LPS + AG compared to LPS group (p < 0.05). Cellular viability showed no statistical difference among groups. Conclusion: This study showed that increased NO/iNOS expression causes an increase in cbfa1 expression in VSMC.
- Published
- 2019