1. Suppressive effect of tranilast on interleukin-5 prolonged eosinophils survival via apoptosis
- Author
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Takashi Ueda, Syunichi Kinjyo, Hirokazu Nakajima, Fukiko Eda, Takeshi Fukuda, Gang Cheng, and Yoshiki Ishii
- Subjects
Cell Survival ,Tranilast ,Inflammation ,Apoptosis ,DNA Fragmentation ,In vivo ,Anti-Allergic Agents ,medicine ,Humans ,ortho-Aminobenzoates ,Fragmentation (cell biology) ,Interleukin 5 ,Cells, Cultured ,Pharmacology ,Electrophoresis, Agar Gel ,business.industry ,Interleukin ,DNA ,respiratory system ,Eosinophil ,Recombinant Proteins ,Eosinophils ,Microscopy, Electron ,medicine.anatomical_structure ,Immunology ,medicine.symptom ,Interleukin-5 ,business ,medicine.drug - Abstract
Tranilast has long been used clinically to treat allergic diseases such as bronchial asthma. To further clarify the antiinflammatory machanism, we examined the ability of tranilast to counteract the prolongation of eosinophil survival induced by interleukin (IL)-5. Tranilast reduced the IL-5 prolonged survival of eosinophils at the concentration range of 30 microg/ml to 100 microg/ml. The DNA extracted from eosinophils cultured with tranilast showed signs of fragmentation that was comparable with apoptosis. Electron-microscopic analysis of activated eosinophils cultured with 100 microg/ml of tranilast also revealed morphologic features of apoptosis. These data suggest that tranilast may act in vivo on activated eosinophils to reduce inflammation in allergic diseases.
- Published
- 2001