Key Points Question What are the reported associations of workplace exclusion and perceived discrimination with employee health behaviors and outcomes in a large academic health care institution? Findings In this qualitative study of 115 participants, most reported outcomes associated with perceived discrimination were emotional (87.8%). More than 1 in 10 narratives (12.2%) described a mental or physical health condition, including stress, depression and anxiety, posttraumatic stress disorder, changes in blood pressure, and changes in sleeping behaviors. Meaning These findings suggest that creating diverse and inclusive environments in which to work and learn can play a vital role in reducing harms associated with perceived discrimination and exclusion., This qualitative study aims to identify and characterize the reported health and well-being outcomes associated with perceived discrimination among academic medicine faculty, staff, and students., Importance Organizational culture and workplace interactions may enhance or adversely impact the wellness of all members of learning and work environments, yet a nuanced understanding of how such experiences within health care organizations impact the health and wellness of their membership is lacking. Objective To identify and characterize the reported health and wellness outcomes associated with perceived discrimination among academic medicine faculty, staff, and students. Design, Setting, and Participants This qualitative study analyzed anonymously submitted written narratives from 2016 that described experiences related to inclusion in the workplace or lack thereof. Narratives that described health outcomes associated with work- or school-based discrimination were purposively sampled. Participants were faculty, staff, and students at health-related schools or hospitals affiliated with the University of Pennsylvania. Data analysis was performed from March 2019 to January 2020. Exposures Self-reported experiences, both witnessed and personal, of discrimination in the workplace. Results A total of 315 narratives were collected, and 115 narratives from 115 participants were analyzed. Most respondents identified as female (70 respondents [60.9%]), non-Hispanic White (68 respondents [59.1%]), and heterosexual (89 respondents [77.4%]) and had worked at the institution for at least 1 year (99 respondents [86.0%]). The outcomes associated with adverse workplace experiences were broad and ranged in nature from emotional to mental and physical. Most reported outcomes were emotional (101 respondents [87.8%]), and more than 1 in 10 narratives (14 respondents [12.2%]) described a mental or physical health outcome. Many of the participants felt devaluated, overexerted, and hopeless, resulting in clinically relevant manifestations, such as increased stress and anxiety levels and even elevated blood pressure. Conclusions and Relevance This qualitative study identified a continuum of negative outcomes on employee health and well-being associated with perceived discrimination and chronic exclusion in the workplace. These findings suggest the need for organizations to promote inclusion as a component of workplace wellness interventions.