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Your search keyword '"Stellato, Francesco"' showing total 6 results
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6 results on '"Stellato, Francesco"'

2. Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Author

Lee, Ho-Hsien, primary, Cherni, Irene, additional, Yu, HongQi, additional, Fromme, Raimund, additional, Doran, Jeffrey D., additional, Grotjohann, Ingo, additional, Mittman, Michele, additional, Basu, Shibom, additional, Deb, Arpan, additional, Dörner, Katerina, additional, Aquila, Andrew, additional, Barty, Anton, additional, Boutet, Sébastien, additional, Chapman, Henry N., additional, Doak, R. Bruce, additional, Hunter, Mark S., additional, James, Daniel, additional, Kirian, Richard A., additional, Kupitz, Christopher, additional, Lawrence, Robert M., additional, Liu, Haiguang, additional, Nass, Karol, additional, Schlichting, Ilme, additional, Schmidt, Kevin E., additional, Seibert, M. Marvin, additional, Shoeman, Robert L., additional, Spence, John C. H., additional, Stellato, Francesco, additional, Weierstall, Uwe, additional, Williams, Garth J., additional, Yoon, Chunhong, additional, Wang, Dingjie, additional, Zatsepin, Nadia A., additional, Hogue, Brenda G., additional, Matoba, Nobuyuki, additional, Fromme, Petra, additional, and Mor, Tsafrir S., additional

Published
2014
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3. Room-temperature macromolecular serial crystallography using synchrotron radiation

Author

Stellato, Francesco, primary, Oberthür, Dominik, additional, Liang, Mengning, additional, Bean, Richard, additional, Gati, Cornelius, additional, Yefanov, Oleksandr, additional, Barty, Anton, additional, Burkhardt, Anja, additional, Fischer, Pontus, additional, Galli, Lorenzo, additional, Kirian, Richard A., additional, Meyer, Jan, additional, Panneerselvam, Saravanan, additional, Yoon, Chun Hong, additional, Chervinskii, Fedor, additional, Speller, Emily, additional, White, Thomas A., additional, Betzel, Christian, additional, Meents, Alke, additional, and Chapman, Henry N., additional

Published
2014
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4. Serial crystallography onin vivogrown microcrystals using synchrotron radiation

Author

Gati, Cornelius, primary, Bourenkov, Gleb, additional, Klinge, Marco, additional, Rehders, Dirk, additional, Stellato, Francesco, additional, Oberthür, Dominik, additional, Yefanov, Oleksandr, additional, Sommer, Benjamin P., additional, Mogk, Stefan, additional, Duszenko, Michael, additional, Betzel, Christian, additional, Schneider, Thomas R., additional, Chapman, Henry N., additional, and Redecke, Lars, additional

Published
2014
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5. In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells.

Author

Jakobi AJ, Passon DM, Knoops K, Stellato F, Liang M, White TA, Seine T, Messerschmidt M, Chapman HN, and Wilmanns M

Abstract

The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. The observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.

Published
2016
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6. Serial crystallography on in vivo grown microcrystals using synchrotron radiation.

Author

Gati C, Bourenkov G, Klinge M, Rehders D, Stellato F, Oberthür D, Yefanov O, Sommer BP, Mogk S, Duszenko M, Betzel C, Schneider TR, Chapman HN, and Redecke L

Abstract

Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized X-ray beam, high-precision diffractometry and shutterless data acquisition with a pixel-array detector, a strategy for collecting data from many micrometre-sized crystals presented to an X-ray beam in a vitrified suspension is demonstrated. By combining diffraction data from 80 Trypanosoma brucei procathepsin B crystals with an average volume of 9 µm(3), a complete data set to 3.0 Å resolution has been assembled. The data allowed the refinement of a structural model that is consistent with that previously obtained using free-electron laser radiation, providing mutual validation. Further improvements of the serial synchrotron crystallography technique and its combination with serial femtosecond crystallography are discussed that may allow the determination of high-resolution structures of micrometre-sized crystals.

Published
2014
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