1. Development of serological assays to identify Helicobacter suis and H. pylori infections
- Author
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Hidenori Matsui, Emiko Rimbara, Masato Suzuki, Kengo Tokunaga, Hidekazu Suzuki, Masaya Sano, Takashi Ueda, Hitoshi Tsugawa, Sohachi Nanjo, Akira Takeda, Makoto Sasaki, Shuichi Terao, Tsuyoshi Suda, Sae Aoki, Keigo Shibayama, Hiroyoshi Ota, and Katsuhiro Mabe
- Subjects
Diagnostic procedure ,Gastroenterology ,Virology ,Science - Abstract
Summary: Helicobacter suis, hosted by hogs, is the most prevalent gastric non-Helicobacter pylori Helicobacter species found in humans. Recent studies have suggested that H. suis infection has caused many cases of gastric disease, but the transmission route from hogs remains unclear. Diagnostic methods based on H. suis urease activity often yield negative results, and there is no reliable method for diagnosing H. suis infection in clinical practice without gastric biopsy specimens. This study presents the world’s first use of whole-bacterial cell ELISA to simultaneously assess H. suis and H. pylori infections. The ELISAs showed high accuracy, with an area under the ROC curve of 0.96, 100% sensitivity, 92.6% specificity, 76.9% positive predictive value, and 100% negative predictive value for the H. suis test, and an area under the ROC curve of 0.92, 88.2% sensitivity, 87.5% specificity, 65.2% positive predictive value, and 96.6% negative predictive value for the H. pylori test.
- Published
- 2023
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