1. Up-Regulation of miR-21, miR-25, miR-93, and miR-106b in Gastric Cancer
- Author
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Alireza Ahadi, Hamid Ghaedi, Mohammad Reza Zali, Pegah Larki, Mojgan Souri, Ali Zare, Mir Davood Omrani, Mahrokh Zaheri, and Shahriar Tarighi
- Subjects
0301 basic medicine ,Adult ,Male ,Prognosis prediction ,Stomach cancer ,Clinical Biochemistry ,Full Length ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Stomach Neoplasms ,microRNA ,medicine ,Biomarkers, Tumor ,Humans ,Mir 106b ,Aged ,Retrospective Studies ,Biochemistry (medical) ,Cancer ,Middle Aged ,medicine.disease ,microRNAs ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Gastric Dysplasia ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Biomarkers - Abstract
Background: Differential expression profile of microRNAs (miRNAs) could be a diagnosis signature for monitoring gastric cancer (GC) progression. In this study, we focus on the comparison of expression levels of miR-21, miR-25, miR-93, miR-106b, and miR-375 during the sequential pattern of GC development, including normal gastric, gastric dysplasia, and GC sample. Methods: We used SYBR Green-based quantitative-PCR to quantify miRNAs expression. Results: Our analysis revealed the increased expression levels of miR-21 (p = 0.034), miR-25 (p = 0.0003), miR-93 (p = 0.0406), and miR-106b (p = 0.023) in GC samples. In addition, GC patients with positive lymph node metastasis showed the up-regulation of miR-25, miR-93, and miR-106b (p < 0.05). Conclusion: Our findings suggested that the expression of miR-21, miR-25, miR-93, and miR-106b altered in GC, and some of them may be further investigated as biomarkers for GC early detection and prognosis prediction.
- Published
- 2018