1. Long Noncoding RNA ZFPM2-AS1 Knockdown Restrains the Development of Retinoblastoma by Modulating the MicroRNA-515/HOXA1/Wnt/β-Catenin Axis.
- Author
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Lyv X, Wu F, Zhang H, Lu J, Wang L, and Ma Y
- Subjects
- Adolescent, Apoptosis genetics, Cell Line, Tumor, Cell Movement, Cell Proliferation, Child, Child, Preschool, Female, Humans, Infant, Male, MicroRNAs metabolism, Nuclear Proteins metabolism, RNA, Long Noncoding metabolism, RNA, Neoplasm genetics, RNA, Neoplasm metabolism, Retinal Neoplasms metabolism, Retinal Neoplasms pathology, Transcription Factors metabolism, beta Catenin biosynthesis, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Nuclear Proteins genetics, RNA, Long Noncoding genetics, Retinal Neoplasms genetics, Transcription Factors genetics, Wnt Signaling Pathway genetics, beta Catenin genetics
- Abstract
Purpose: The tumor-initiating function of long non-coding RNA (lncRNA), zinc finger protein multitype 2 antisense RNA 1 (ZFPM2-AS1) was reported in lung cancer, yet the relevance of ZFPM2-AS1 in retinoblastoma (RB), a malignancy representing 2.5% to 4% incidence of cancers among children, has not been elucidated. Thus, we attempted to assess the effect of ZFPM2-AS1 and underlying mechanism in RB progression., Methods: First, comparing the differentially expressed lncRNAs in normal retinal tissues as well as RB tissues, the target lncRNA ZFPM2-AS1 was screened out. We then assayed the ZFPM2-AS1 expression in three RB cell lines, and carried out methylthiazol tetrazolium (MTT), transwell assays, and flow cytometric analyses to examine the role of si-ZFPM2-AS1 on cell behaviors. Following online database predication, the correlations between ZFPM2-AS1 and microR-515 (miR-515) or homeobox A1 (HOXA1) were corroborated by dual-luciferase reporter gene assays. Quantitative real-time PCR along with Western blot assays was fulfilled to ascertain the expression of relevant genes., Results: ZFPM2-AS1 was significantly overexpressed in RB tissues and cell lines, and ZFPM2-AS1 silencing curtailed the growth and metastasis of RB cells both in vitro and in vivo. Bioinformatic websites and dual-luciferase reporter gene assays disclosed that ZFPM2-AS1 might perform as a competing endogenous RNA for miR-515 and positively correlate with HOXA1 to activate the Wnt/β-catenin signaling pathway., Conclusion: Altogether, these data demonstrated that ZFPM2-AS1 interacted with HOXA1 to promote RB development via mediating miR-515, establishing a promising therapeutic biomarker for RB and prognosis.
- Published
- 2020
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