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Byline: Maik Kilian (1), Jan Ilga Gregor (1), Ina Heukamp (2), Chris Braumann (1), Hans Guski (3), Ingolf Schimke (4), Martin Karl Walz (5), Christoph Andreas Jacobi (1), Frank Axel Wenger (5,6) Keywords: octreotide; taurolidin; lavage; laparoscopy; metastasis Abstract: Background: There is controversial discussion whether metastasis initiated by laparoscopy with carbon dioxide might be prevented by instillation of taurolidin or radical scavengers like the somatostatin analogue Octreotide. Therefore we evaluated the effects of laparoscopic lavage with taurolidin and Octreotide on liver metastasis after staging laparoscopy in ductal pancreatic cancer. Methods: In 60 Syrian hamsters pancreatic adenocarcinoma was induced by weekly subcutanous injection of 10 mg N-nitrosobis-2-oxopropylamin/kg body weight for 10 weeks. In the 16th week laparoscopic staging biopsy by use of carbon dioxide was performed. Finally animals underwent abdominal irrigation with saline (gr.1, n = 20), taurolidin (0.5%) (gr.2, n = 20) or Octreotide (gr.3, n = 20). In week 25 animals were sacrificed, pancreas and liver were analysed. Results: Size of pancreatic carcinomas was decreased in the taurolidin gr. compared to the other two groups. Furthermore the number of liver metastasis per animal was reduced after lavage with taurolidin (2 +- 2) and Octreotide (2.5 +- 2) compared to saline irrigation (4 +- 4) (p &lt 0.05). Additionally the incidence of port site metastases was significantly reduced in the taurolidin group. Activity of antioxidative enzyme superoxide dismutase (SOD) was increased while concentration of products of lipidperoxidation was decreased in non-metastatic liver after taurolidin irrigation compared to saline or Octreotide irrigation. Conclusions: Taurolidin irrigation during laparoscopy might be a new concept to reduce the number of liver metastasis and port site metastases in pancreatic cancer. Author Affiliation: (1) Clinic of General, Visceral, Vascular and Thoracic Surgery, Humboldt-University, Schumannstr. 2/21, 10117, Berlin, Germany (2) Clinic of Hepatology, Gastroenterology and Endocrinology, Humboldt-University, Schumannstr. 2/21, 10117, Berlin, Germany (3) Institute of Pathology, Humboldt-University, Schumannstr. 2/21, 10117, Berlin, Germany (4) Clinic of Internal Medicine I, Charite Campus Mitte, Humboldt-University, Schumannstr. 2/21, 10117, Berlin, Germany (5) Clinic of Surgery and Center of Minimal-invasive Surgery, Huyssens-Stiftun/Knappschafts-GmbH, Kliniken Essen-Mitte, Henricistrasse 92, 45136, Essen, Germany (6) Clinic of Surgery and Center of Minimal-Invasive Surgery, Huyssens-Stiftun/Knappschafts-GmbH, Kliniken Essen-mitte, Henricistrasse 92, 45136, Germany Article History: Registration Date: 01/01/2005