1. Activation of Eukaryotic Transcriptional Promoters by the Bovine Papillomavirus E1-Replication Factor
- Author
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Paolo Monini, Moataza Hassan-Omran, Paola Borgatti, L. De Lellis, and Enzo Cassai
- Subjects
Chloramphenicol O-Acetyltransferase ,Transcription, Genetic ,Activator (genetics) ,Recombinant Fusion Proteins ,viruses ,DNA replication ,Promoter ,Biology ,Transfection ,biology.organism_classification ,Molecular biology ,DNA-Binding Proteins ,DNA replication factor CDT1 ,Viral Proteins ,Infectious Diseases ,Plasmid ,Virology ,Gene expression ,biology.protein ,Humans ,Heterologous expression ,Promoter Regions, Genetic ,Bovine papillomavirus 1 ,HeLa Cells ,Bovine papillomavirus - Abstract
It has been suggested that the bovine papillomavirus type 1 E1 replication factor may inhibit E2-conditional gene expression from viral promoter P89. To study the possible role of the E1 protein in gene expression, HeLa cells were transfected with E2-conditional or E2-independent reporter plasmids and with vectors expressing the E1 and E2 open reading frames. The data show that: (i) replication factor E1 stimulates gene expression from a variety of eucaryotic transcriptional promoters; (ii) activation of gene expression by E1 also occurs in the absence of viral activator E2-TA; (iii) expression of a recombinant plasmid containing the human papillomavirus type 18 origin of DNA replication is stimulated, but not repressed, by E1, and (iv) E1-dependent activation of gene expression does not reflect the amplification of transfected plasmids.
- Published
- 1993
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