1. A monoclonal antibody that neutralizes Epstein-Barr virus DNA polymerase activity.
- Author
-
Tsai CH, Williams MV, and Glaser R
- Subjects
- Animals, Antibodies, Monoclonal biosynthesis, Antibody Specificity, Antigens, Viral immunology, Blotting, Western, Cell Line, Fluorescent Antibody Technique, Herpesvirus 4, Human enzymology, Mice, Mice, Inbred BALB C, Molecular Weight, Neutralization Tests, Nucleic Acid Synthesis Inhibitors, Antibodies, Monoclonal immunology, DNA-Directed DNA Polymerase immunology, Herpesvirus 4, Human immunology
- Abstract
An Epstein-Barr virus (EBV) specific monoclonal antibody (MAb), designated 55H3, was produced after immunizing BALB/c mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) activated B95-8 cells. It was possible to demonstrate that this MAb detected an EBV-specific antigen(s) in the EBV genome-positive producer cell lines B95-8 and M-ABA by immunofluorescence. Immunoglobulin prepared from ascites fluid neutralized the activity of the EBV-encoded DNA polymerase, but not the alkaline DNase. By Western blotting, the 55H3 MAb reacts with an 85/80-KD polypeptide. The 55H3 should be useful in examining the role of EBV DNA polymerase in viral replication.
- Published
- 1990
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