1. Isolation and Characterization of Densonucleosis Virus from Aedes aegypti Mosquitoes and Its Distribution in India.
- Author
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Sivaram, A., Barde, P. V., Kumar, S. R. P., Yadav, P., Gokhale, M. D., Basu, A., and Mourya, D. T.
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MOSQUITO vectors , *CELL lines , *MORTALITY , *VIRUS isolation , *WESTERN immunoblotting - Abstract
Objectives: Mosquito densonucleosis viruses (DNVs) are known to persistently infect the insect cell line and mosquito population in nature, causing mortality in mosquitoes. Here we report the isolation and characterization of a DNV from Aedes aegypti and its distribution among different Ae. aegypti populations from India. Methods: We screened Ae. aegypti mosquito populations from different states of India by PCR. Virus isolation and purification was performed using a cesium chloride gradient from a positive mosquito colony. Characterization of this isolate was carried out by electron microscopy, Western blot and sequencing. Results: Electron microscopy showed the presence of parvovirus-like particles, and Western blot showed the presence of 2 viral proteins of 40 and 41 kDa. A total of 3,776 bases of genome were sequenced, which included a 3′UTR of 128 bases, a coding region of 3,507 bases and a 5′UTR of 141 bases. Three open reading frames (ORFs) were identified and characterized. The NIVDNV genome showed 95% similarity with Culex pipiens pallens DNV and 93% similarity with Ae. aegypti DNV. Conclusion: Phylogenetic analysis of all 3 ORFs showed that this new isolate falls in the lineage of Brevidensovirus along with other mosquito DNVs. Copyright © 2009 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2009
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