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Two- and three-dimensional direct numerical simulation (DNS) of turbine cascade flow at low Reynolds number with heat transfer are performed using high-order finite difference method. Two-dimensional laminar computation which is used to construct the initial flow of three-dimensional DNS fails to predict Stanton number on the second half of suction side where the flow is turbulent in experiment. In three-dimensional DNS, transition is triggered by periodic blow-and-suction disturbances. Numerical experiments show that phase randomness of the disturbance is not necessary to trigger the transition which can be induced by disturbances with fixed phases. In a range of time fundamental frequency of disturbance, when increasing the frequency, transition moves downstream. When time fundamental frequency is big enough, transition disappears. With fixed space phases, time phases and selected time fundamental frequency, time averaged pressure and Stanton number distributions of three-dimensional DNS coincide with the experimental datum. Averaged velocity and temperature, Root-Mean-squares (RMS) of velocity pulse，temperature pulse, Reynolds shear stress and heat flux are extracted from the DNS database. All statistics agree well with experimental and theoretical results which verify the accuracy of present database. [ABSTRACT FROM AUTHOR]