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Panonychus citri (citrus red mite) is a devastating pest of citrus orchards. The conventional chemical acaricides have been strongly forbidden for the management of agricultural insect pests in China. Therefore, we evaluated the susceptibility of adult and nymphs P. citri in laboratory against eight isolates of four fungal species, Akanthomyces lecanii, Metarhizium anisopliae, Beauveria bassiana and Aschersonia aleyrodis. Each citrus seedling having 40 adults (2-d-old) and nymphs (on separate plants) were sprayed with isolates at the concentration of 104 ~ 108 conidia mLˉ1 whereas controlled seedlings were sprayed with 0.02% Tween-80. After 9 days of fungal exposure, the four fungal isolates caused more than 50% mortality of mites, such as; 85.6%, 87.9%, 64.6% and 79.7% by A. lecanii (V3450), B. bassiana (BFZ0409), M. anisopliae (MFZ0706) and A. aleyrodis (AsG0910), respectively. The nymphal mites were less susceptible to applied fungi compared to adults. The LC50s of the tested isolates were determined by the fitted time-concentration-mortality relationships, which declined over days after spray. LT50s were decreased with a high concentration of isolates. After the 9-d inoculation, two isolates of B. bassiana (BFZ0409 and D1344) and one isolate of A. lecanii (V3450) were highly effective at the minimal dose of LC50 of 104 conidia mLˉ1 and are promising candidates to control mites, as compared to other tested fungal isolates.