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A Gram-negative, moderately halophilic, strictly aerobic strain, designated YIM 95345T, was isolated from a soil sample of a hypersaline mine in Yunnan province, PR China, and subjected to a polyphasic taxonomic study. Strain YIM 95345T grew at 15–45 °C (optimum 30–35 °C), 3.0–23.0 % (w/v) NaCl (optimum 10.0–11.0 %, w/v) and pH 6.0–9.0 (optimum pH 7.0–8.0). Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism belongs to the genus Aquisalimonas and exhibited sequence similarity of 96.6 % to the sole type strain Aquisalimonas asiatica CG12T. The predominant isoprenoid quinone was Q-8 and the major fatty acids were C16 : 0, C19 : 0 cyclo ω8c and C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three aminolipids and three unidentified phospholipids. The G+C content of the genomic DNA was 59.4 mol%. Based on the results of our comparative phylogenetic, chemotaxonomic and physiological analyses, the new isolate is assigned to a novel species of the genus Aquisalimonas , for which the name Aquisalimonas halophila sp. nov. is proposed, with the type strain YIM 95345T ( = DSM 25902T = CCTCC AB 2012043T).

A bacterial strain, RS-LYSO-3T, was isolated from tobacco-cultivated soil, collected near Chuxiong, Yunnan province, southwestern China. RS-LYSO-3T could effectively inhibit the invasion of powdery mildew on tobacco. The colonies of RS-LYSO-3T were pale yellow, and its cells were Gram-stain-negative and rod-shaped, with 68 mol% DNA G+C content. Gene sequence analysis for its 16S rRNA gene revealed the highest similarity (97.78 %) with that of Lysobacter spongiicolaKMM 329T. Chemotaxonomic data showed that RS-LYSO-3T possesses a quinone system with Q-8, and iso-C16 : 0, summed feature 9 and iso-C15 : 0 as the predominant fatty acids, all of which support the affiliation of RS-LYSO-3T to the genus Lysobacter . The results of DNA–DNA hybridization, physiological and biochemical tests clearly proved that RS-LYSO-3T is a representative of a novel species of the genus Lysobacter , for which the name Lysobacter erysipheresistens sp. nov. is proposed. The type strain is RS-LYSO-3T (=CCIC 23922T=JCM 31042T).

A Gram-stain-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161T, was isolated from brine of a salt well in Yunnan province, China, and subjected to a taxonomic study using a polyphasic approach. Cells of strain YIM 95161T were short rods, approximately 0.9–1.4 µm long and 0.4–0.6 µm wide. Strain YIM 95161T grew at 15–40 °C (optimum, 25–30 °C), 6–29 % (w/v) NaCl (optimum, 14–19 %) and at pH 5.0–8.0 (optimum, pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C14 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown phosphoglycolipid and two unknown lipids. The DNA G+C content was 69.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 95161T was a member of the genus Salinisphaera and exhibited sequence similarities of 96.7 %, 95.6 % and 95.4 % to Salinisphaera shabanensis E1L3AT, Salinisphaera dokdonensis CL-ES53T and Salinisphaera hydrothermalis EPR70T, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analysis, strain YIM 95161T represents a novel species of the genus Salinisphaera , for which the name Salinisphaera halophila sp. nov. is proposed. The type strain is YIM 95161T ( = CCTCC AB 2011132T = JCM 17431T).

A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, strain A-1T, was isolated from a saline soil contaminated with crude oil in Xianhe, Shangdong Province, China. Strain A-1T formed yellow colonies, was moderately halophilic and grew with 0.05–27.5 % (w/v) total salts (optimum 5–8 %), at 10–42 °C (optimum 30 °C) and at pH 5.5–9.0 (optimum pH 7.2). The dominant fatty acids (>5 %) were C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c, C19 : 0 cyclo ω8c and C12 : 0 3-OH and the predominant ubiquinone was Q-9. The genomic DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-1T belonged to the genus Halomonas in the class Gammaproteobacteria. The closest relatives were Halomonas lutea YIM 91125T (97.7 % 16S rRNA gene sequence similarity), H. muralis LMG 20969T (95.6 %), H. pantelleriensis AAPT (95.5 %) and H. kribbensis BH843T (95.2 %). DNA–DNA relatedness between strain A-1T and H. lutea CCTCC AB 206093T was 27±3 %. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain A-1T should be placed in the genus Halomonas as a representative of a novel species. The name Halomonas xianhensis sp. nov. is proposed, with strain A-1T ( = CGMCC 1.6848T = JCM 14849T) as the type strain.

A novel Gram-stain-positive, motile, strictly aerobic bacterium, designated YIM 93174T, was isolated from a salt field in Korea. Cells of this strain were rod-shaped and formed pale tangerine colonies and grew at pH 6.0–8.0 (optimal growth at pH 7.0), at 15–45 °C (optimum 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimum 0–2 % NaCl). Some phenotypic characters allowed differentiation of strain YIM 93174T from its nearest phylogenetic relatives. Comparative 16S rRNA gene sequence analysis showed that strain YIM 93174T belongs to the genus Bacillus, exhibiting the highest level of sequence similarity with the type strain of Bacillus humi (95.7 %), followed by those of Bacillus alkalitelluris (94.9 %) and Bacillus litoralis (94.5 %). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The cell-wall peptidoglycan was of the A1γ type, containing meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 36.9 mol% and the predominant respiratory quinone was MK-7. The major polar lipids of strain YIM 93174T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unknown phospholipids. On the basis of the evidence from this polyphasic study, strain YIM 93174T represents a novel species of the genus Bacillus, for which the name Bacillus luteolus sp. nov. is proposed, with YIM 93174T ( = DSM 22388T = KCTC 13210T = CCTCC AA 208068T) as the type strain.