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Strain RA15 T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15 T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4-32 °C and at pH values of 5.0-9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C 16 : 1   ω7 c and/or C 16 : 1   ω6 c ), C 17 : 1  ω 8 c and C 16 : 0 . The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15 T within the genus Pseudoalteromonas , close to the type strains of Pseudoalteromonas neustonica , Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa . Results of comparative phylogenetic and phenotypic studies between strain RA15 T and its closest related species suggest that RA15 T could be a new representative of the genus Pseudoalteromonas , for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15 T (=CECT 9079 T =LMG 29860 T ). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.

Strain EAR18 T was isolated as an endophyte from the roots of a halophyte plant, Arthrocnemum macrostachyum , growing in the Odiel marshes (Huelva, Spain). Cells of strain EAR18 T were Gram- stain-negative, motile, non-spore-forming aerobic rods. It grew optimally on tryptic soy agar supplemented with 2.5 % NaCl (w/v), at pH 7 and 30 °C for 48 h. It tolerated NaCl from 0 to 25 % (w/v). It presented Q9 as the major quinone and C 19 : 0 cyclo ω 8 c , summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and C 16 : 0 as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified phospholipids. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EAR18 T formed a well-supported clade with species Halomonas zincidurans B6 T and Halomonas xinjiangensis TRM 0175 T (similarities of 98.3 and 96.1 % respectively). Furthermore, digital DNA-DNA hybridization analysis resulted in values of 20.4 % with H. xinjiangensis TRM 0175 T and 35.50 % with H. zincidurans B6 T , and ANIb/ANIm results in values of 73.8 %/84.2 % with H. xinjiangensis TRM 0175 T and 86.8 %/89.4 % with H. zincidurans B6 T . Based on phylogeny and differential phenotypic properties in comparison with its closest related species, strain EAR18 T is suggested to represent a new species in the genus Halomonas , for which the name Halomonas radicis sp. nov. is proposed. The type strain is EAR18 T (=CECT 9077 T =LMG 29859 T ). The whole genome was sequenced, and it had a total length of 4.6 Mbp and a G+C content of 64.9 mol%.

Two endophytic bacteria (EAod3 T and EAod7 T ) were isolated from the aerial part of plants of Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). Phylogenetic analysis based on 16S rRNA gene sequences indicated their affiliation to the genus Kushneria. 16S rRNA gene sequences of strains EAod3 T and EAod7 T showed the highest similarity to Kushneria marisflavi DSM 15357T (99.0 and 97.6 %, respectively). Digital DNA-DNA hybridization studies between the draft genomes of strain EAod3 T and K. marisflavi DSM 15357 T corresponded to 28.5 % confirming the novel lineage of strain EAod3 T in the genus Kushneria. Cells of both strains were Gram-staining-negative, aerobic and motile rods able to grow at 4-37 °C, at pH 5.0-8.0 and tolerate 0.5-25 % NaCl (w/v). They presented ubiquinone Q9 and C16 : 0, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c as the major fatty acids. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the phenotypic and phylogenetic results, strains EAod3 T (=CEC T 9073 T =LMG 29856 T ) and EAod7 T (=CECT 9075 T =LMG 29858 T ) are proposed as new representatives of the genus Kushneria, and the proposed names are Kushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., respectively. The whole genome sequence of strain EAod3 T has a total length of 3.8 Mbp and a G+C content of 59.3 mol%.