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A novel Vogesella strain, YM-1 T , was recovered from human urine in PR China in 2017. Cells of strain YM-1 T were Gram-stain-negative, rod-shaped, aerobic, motile, non-spore-forming and poly-β-hydroxybutyrate-accumulating. The strain contained C 16:1 ω6 c /C 16:1 ω7 c , C 16:0 and C 18:0 ω7 c as major fatty acids; phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid as major polar lipids; and ubiquinone-8 as the predominant respiratory quinone. Comparison of 16S rRNA gene sequences indicated that this strain had highest similarities to Vogesella perlucida DS-28 T (98.8 %) and Vogesella mureinivorans 389 T (98.1 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel strain was clustered and well separated with V. perlucida DS-28 T and V. mureinivorans 389 T within the genus Vogesella . The average nucleotide identity (ANI) and amino acid identity (AAI) analyses showed that this strain was not identified as V. perlucida DS-28 T or V. mureinivorans 389 T , with values well below the threshold limit for species demarcation (ANI <88.1 %, AAI <88.6 %). Based on the above results, strain YM-1 T is proposed to be a novel species of the genus Vogesella with the name Vogesella urethralis sp. nov. (YM-1 T =NBRC 113779=CGMCC 1.17135).

A Gram-stain-negative, aerobic, non-endospore-forming, motile by a polar flagellum, rod-shaped bacterium, designated strain DHOG02 T , which produced yellow-pigmented colonies, was isolated from a soil sample collected from the lower subtropical forest of the Dinghushan Biosphere Reserve, Guangdong Province, PR China. Strain DHOG02 T grew at 12-37 °C, pH 4-9 and 0-4 % (w/v) NaCl, with optima at 28 °C, pH 6-7 and 0.5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain formed a clade with Dyella lipolytica DHOB07 T and Dyella jejuensis JP1 T , with sequence similarities of 98.0 and 97.4 %, respectively. The result of the concatenated partial gyrB, lepA and recA gene sequence analysis confirmed that strain DHOG02 T belongs to the genus Dyella, but is distinct from all currently known species of the genus. The G+C content of the genomic DNA was 62 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and phospholipid. Ubiquinone-8 was the only respiratory quinone detected, and iso-C15 : 0, iso-C17 : 1ω9c and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) were the major fatty acids, all of which supported the affiliation of strain DHOG02 T to the genus Dyella. On the basis of the evidence presented here, strain DHOG02 T represents a novel species of the genus Dyella, for which the name Dyella halodurans sp. nov. is proposed. The type strain is DHOG02 T (=NBRC 111474 T =CGMCC 1.15435 T ).

Two strains, 4G-K17 T and 4G-K15, were isolated from forest soil from the Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). The cells of the two strains were Gram-stain-negative, aerobic and non-motile short rods that multiplied by binary division. Strains 4G-K17 T and 4G-K15 were obligately acidophilic, mesophilic bacteria capable of growth at pH 3.0-7.0 (optimum 4.0-5.5 and 3.5-5.5, respectively), temperature 12-42 °C (optimum 28 °C) and NaCl concentrations from 0~2.5 % and 0~3.0 % (w/v), respectively. They had a 16S rRNA gene sequence similarity of 99.2 % and showed the highest similarities of 97.1 and 97.4 % to Edaphobacter aggregans Wbg-1 T , respectively, which indicated that these two isolates belonged to a novel species of the genus Edaphobacter in subdivision 1 in the family Acidobacteriaceae. The DNA G+C contents of strains 4G-K17 T and 4G-K15 were 57.6 and 57.2 %, respectively. They had similar fatty acid profiles, with the major (>10 %) fatty acid profile comprising iso-C15 : 0, C16 : 0 and summed feature 3 (iso-C16 : 1ω7c and/or C16 : 1ω6c), and the major polar lipid profile comprising phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified aminophospholipids and unidentified phospholipids. All physiological, phylogenetic and chemotaxonomic data suggest that strains 4G-K17 T and 4G-K15 belong to a new species of the genus Edaphobacter, for which the name Edaphobacter acidisoli sp. nov. is proposed, with 4G-K17 T (=CGMCC 1.15447=LMG 29212) as the type strain.