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Aim This study compared the effect of different resistance training (RT) frequencies on total, android, gynoid and trunk body fat in overweight/obese older women. Methods Fifty-seven overweight/obese older women (66.9 ± 5.3 years and 39.9 ± 4.9 % body fat) were randomly assigned to one of three groups: a group performing RT twice a week (G2X), a group performing RT three times a week (G3X), or a non-exercise control group (CG). Both training groups performed the same 12-week RT program consisting of 8 exercises that trained all major muscle groups. Dual-energy X-ray absorptiometry was used to assess body composition. Results After the intervention period, both G2X and G3X demonstrated significant (P < 0.05) reductions in adiposity compared to the CG for total body fat (G2X = --1.7 %, G3X = --2.7 %, CG = + 2.1 %), android fat (G2X = --6.2 %, G3X = --7.0 %, CG = + 8.6 %), gynoid fat (G2X = --2.5 %, G3X = --2.9 %, CG = + 1.0 %), and trunk fat (G2X = --2.5 %, G3X = --3.0 %, CG = + 2.9 %), with no significant differences between training groups. Conclusion These results demonstrate that a low-volume 12- week RT program performed two or three times per week causes decreases in total and regional fat deposition with the greatest reductions occurring in the android region. [ABSTRACT FROM AUTHOR]