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Background: Bullying among adolescences is known as a public health abnormality and studying the prevalence of bullying behaviors using different methods of data gathering can help researchers in surveillance and planning preventions. Objectives: The objective of this study was to compare the prevalence of perpetration and victimization of bullying reported by self-report, peer-report, and principle-report. Methods: In this cross-sectional study that was conducted in 2017 - 2018, a total of 1,540 students from 42 schools throughout Mazandaran province completed self- and peer-report questionnaires and 42 principals of 42 schools completed a principal-report questionnaire. The chi-square test was used to compare the prevalence of victimization and perpetration of bullying with self-report, peer-report, and principal-report methods across gender, school types and grade levels. Results: The prevalence of bullies and victims in self-, peer-, and principal-report methods were different (20.2% as victim and 7.8% as a bully for self-report, 9.4% as victim and 8.9% as a bully for peer-report, and 4.3% as victim and 3.1% as a bully for principal-report). Conclusions: This study showed the discrepancy in the prevalence of perpetration and victimization of bullying reported in three measurement methods (self-, peer-, and principal-report) in Iranian schools and suggests the use of multiple assessment methods for bullying behaviors.