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Leaf Area Index (LAI) remains one of the most important forest structural attributes, as accurate estimation of LAI is crucial for predicting the growth of different species. Using the Partial Least Squares Regression (PLS-R) algorithm, this study investigated three image processing techniques to determine the best technique for estimating LAI using WorldView-3 imagery in the Midlands, KwaZulu-Natal province of South Africa. The PLS-R texture ratios model achieved the highest accuracy of R2 = 0.70, RMSE = 1.21 (2.32% of the mean measured LAI) and R2 = 0.72, RMSE = 1.26 (2.41% of the mean measured LAI) for the wet and dry seasons, respectively. This was followed by the PLS-R single texture band model that produced an accuracy of R2 = 0.65, RMSE = 1.35 (2.58% of the mean measured LAI) and R2 = 0.67, RMSE = 1.32 (2.52% of the mean measured LAI) for the wet and dry seasons, respectively. The PLS-R model using a combination of vegetation indices had the lowest estimation accuracy of R2 = 0.59, RMSE = 1.38 (2.64% of the mean measured LAI) and R2 = 0.60, RMSE = 1.40 (2.67% of the mean measured LAI) for the wet and dry seasons, respectively. The results of this study provided evidence that image texture ratios can be used to estimate LAI effectively. [ABSTRACT FROM AUTHOR]