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Your search keyword '"Gomez *, Rafael"' showing total 6 results
Start Over Author "Gomez *, Rafael" Journal international journal of pharmaceutics
6 results on '"Gomez *, Rafael"'

6. Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction

Author

Rafael Gomez-Ramirez, Francisco Javier de la Mata, Svetlana Loznikova, Alya G. Venyaminova, Inessa Halets, Jean-Pierre Majoral, Dzmitry Shcharbin, Katarzyna Milowska, D. S. Novopashina, Volha Dzmitruk, Maksim Ionov, Joanna Lazniewska, Olga A. Krasheninina, Maria Bryszewska, Evgeny K. Apartsin, Olga Nowacka, Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, University of Lódź, Institute of Biophysics and Cell Engineering, NASB, Minsk, National Academy of Sciences of Belarus (NASB), Institute of of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Institute of Chemical Biology and Fundamental Medicine, Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá, Alcalá de Henares, Universidad de Alcalá - University of Alcalá (UAH), Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III [Madrid] (ISC)-ministerio de ciencia e innovacion, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ionov, Maksim, Lazniewska, Joanna, Dzmitruk, Volha, Halets, Inessa, Loznikova, Svetlana, Novopashina, Darya, Apartsin, Evgeny, Krasheninina, Olga, Venyaminova, Alya, Milowska, Katarzyna, Nowacka, Olga, Gomez-Ramirez, Rafael, Javier de la Mata, Francisco, Majoral, Jean-Pierre, Shcharbin, Dzmitry, and Bryszewska, Maria

Subjects
Dendrimers, Small interfering RNA, Circular dichroism, Light, RNase P, Stereochemistry, anticancer siRNA, biophysical characteristics, bcl-X Protein, Pharmaceutical Science, 02 engineering and technology, dendrimer, Transfection, 03 medical and health sciences, Microscopy, Electron, Transmission, complex formation, Neoplasms, Dendrimer, Zeta potential, Humans, Scattering, Radiation, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Pharmacology & Pharmacy, Particle Size, RNA, Small Interfering, 030304 developmental biology, Electrophoresis, Agar Gel, 0303 health sciences, Heparin, Chemistry, Circular Dichroism, Phosphorus, Silanes, 021001 nanoscience & nanotechnology, 3. Good health, Gene Expression Regulation, Neoplastic, RNAi Therapeutics, Spectrometry, Fluorescence, Proto-Oncogene Proteins c-bcl-2, Cell culture, Cancer cell, Biophysics, Myeloid Cell Leukemia Sequence 1 Protein, Nucleic Acid Conformation, Nanocarriers, 0210 nano-technology, nano-drug-delivery
Abstract

International audience; This paper examines a perspective on the use of newly engineered nanomaterials as effective and safe carriers of genes for the therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus and carbosilane) were complexed with anticancer siRNA and their biophysical properties of the dendriplexes analyzed. The potential of the dendrimers as nanocarriers for anticancer siBcl-xl, siBcl-2, siMcl-1 siRNAs and a siScrambled sequence was explored. Dendrimer/siRNA complexes were characterized by methods including fluorescence, zeta potential, dynamic light scattering, circular dichroism, gel electrophoresis and transmission electron microscopy. Some of the experiments were done with heparin to check if siRNA can be easily disassociated from the complexes, and whether released siRNA maintains its structure after interaction with the dendrimer. The results indicate that siRNAs form complexes with all the dendrimers tested. Oligoribonucleotide duplexes can be released from dendriplexes after heparin treatment and the structure of siRNA is maintained in the case of PAMAM or carbosilane dendrimers. The dendrimers were also effective in protecting siRNA from RNase A activity. The selection of the best siRNA carrier will be made based on cell culture studies (Part B).

Published
2015

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