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A descriptive study was conducted to assess the occupational stress among nurses working in Government and private sector in Kottayam District, Kerala. The research study was undertaken in one government medical college hospital and two private hospitals of Kottayam district, Kerala, India. Sample consists of 400 nurses selected by simple random sampling technique, 200 each from the government and private sector. The Tools used for the study were socio-personal data sheet and a stress assessment rating scale. The maximum score of the stress assessment rating scale was 448 which is categorized as mild stress (1–149), moderate stress (150–299) and severe stress (300–448). All the staff nurses in the present study experienced stress ranging from mild to severe. The findings showed that the mean stress score of the nurses working in government sector was significantly higher than that of the nurses working in private sector (t=2.52, p