27 results on '"Fang, Jia"'
Search Results
2. Proteinase K/Retinoic Acid-Loaded Cationic Liposomes as Multifunctional Anti-Acne Therapy to Disorganize Biofilm and Regulate Keratinocyte Proliferation
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Tsai, Ming-Jun, primary, Lin, Cheng-Yu, additional, Trousil, Jiří, additional, Sung, Calvin T, additional, Lee, Mei-Hua, additional, Fang, Jia-You, additional, and Yang, Shih-Chun, additional
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- 2023
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3. Self-Nanoemulsifying Drug Delivery System-Containing the Poorly Absorbed Drug – Valsartan in Post-Bariatric Surgery
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Huang, Tzu-Hao, primary, Chen, Chih-Jung, additional, Lin, Hsin-Chia Angela, additional, Chen, Chun-Han, additional, and Fang, Jia-You, additional
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- 2023
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4. A TA/Cu2+ Nanoparticle Enhanced Carboxymethyl Chitosan-Based Hydrogel Dressing with Antioxidant Properties and Promoting Wound Healing.
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Huang, Yongjun, Chen, Yong, Cheng, Guoyun, Li, Wenqiang, Zhang, Hongan, Yu, Chaoqun, Fang, Jia, Zuo, Jieyi, Li, Ying, Xu, Lei, and Sun, Dawei
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- 2024
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5. The Cleaning Effect of the Photocatalysis of TiO2-B@anatase Nanowires on Biological Activity on a Titanium Surface
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Ying Liu, Zehong Guo, Chunhua Lai, Xi Lin, Xianglong Ding, Yadong Zhao, Shulan Xu, Lei Zhou, Wangxi Wu, Yan Gao, and Fang Jia
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Anatase ,Materials science ,Organic Chemistry ,Biophysics ,Nanowire ,Pharmaceutical Science ,chemistry.chemical_element ,Bioengineering ,02 engineering and technology ,General Medicine ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Cell morphology ,01 natural sciences ,Osseointegration ,0104 chemical sciences ,Biomaterials ,chemistry ,Chemical engineering ,Drug Discovery ,Photocatalysis ,Degradation (geology) ,0210 nano-technology ,Photodegradation ,Titanium - Abstract
Background Improvements in the early osseointegration of titanium implants require investigations on the bone-implant interface, which is a critical and complex challenge. The surface cleanliness of titanium implants plays an important role at this interface. However, the implant surface would inevitably absorb contamination such as organic hydrocarbons, which is not conductive to the establishment of early osseointegration. Herein, an optimized approach for removing contamination from titanium surfaces was studied. Methods The TiO2-B@anatase NWs (nanowires) were prepared on titanium substrates through a hydrothermal process. A methylene blue degradation experiment was performed to assess the photodegradation activity. The cleaning effect of the photocatalysis of TiO2-B@anatase NWs on a titanium surface and the cellular early response was determined by analyzing cell morphology, attachment, proliferation and differentiation. Results The results indicated that the photocatalysis of TiO2-B@anatase NWs could effectively remove hydrocarbons on titanium surfaces without sacrificing the favourable titanium surface morphology. The methylene blue degradation experiment revealed that the photocatalysis of TiO2-B@anatase NWs had powerful degradation activity, which is attributed to the presence of strong oxidants such as ·OH. In addition, compared to the merely ultraviolet-treated titanium surfaces, the titanium surfaces treated after the NWs photocatalytic cleaning process markedly enhanced cellular early response. Conclusion The photocatalysis of TiO2-B@anatase NWs for the removal of contamination from titanium surfaces has the potential to enable the rapid and complete establishment of early osseointegration.
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- 2020
6. Facile Biofilm Penetration of Cationic Liposomes Loaded with DNase I/Proteinase K to Eradicate Cutibacterium acnes for Treating Cutaneous and Catheter Infections
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Fang, Jia-You, primary, Chou, Wei-Ling, additional, Lin, Chwan-Fwu, additional, Sung, Calvin T, additional, Alalaiwe, Ahmed, additional, and Yang, Shih-Chun, additional
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- 2021
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7. The Droplet-Size Effect Of Squalene@cetylpyridinium Chloride Nanoemulsions On Antimicrobial Potency Against Planktonic And Biofilm MRSA
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Fang,Jia-You, Lin,Yin-Ku, Wang,Pei-Wen, Alalaiwe,Ahmed, Yang,Yu-Ching, and Yang,Shih-Chun
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Methicillin-Resistant Staphylococcus aureus ,Proteomics ,Squalene ,nanoemulsion ,Cetylpyridinium ,MRSA ,Microbial Sensitivity Tests ,Plankton ,biofilm ,Anti-Bacterial Agents ,Anti-Infective Agents ,International Journal of Nanomedicine ,size effect ,Biofilms ,Cations ,cetylpyridinium chloride ,Humans ,Nanoparticles ,Emulsions ,Micelles ,Original Research - Abstract
Jia-You Fang,1–4,* Yin-Ku Lin,5,6,* Pei-Wen Wang,7 Ahmed Alalaiwe,8 Yu-Ching Yang,1 Shih-Chun Yang9 1Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan; 2Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Kweishan, Taoyuan, Taiwan; 3Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan; 4Department of Anesthesiology, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan; 5School of Traditional Chinese Medicine, Chang Gung University, Kweishan, Taoyuan, Taiwan; 6Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital at Keelung, Keelung, Taiwan; 7Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan; 8Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al Kharj, Saudi Arabia; 9Department of Cosmetic Science, Providence University, Taichung, Taiwan*These authors contributed equally to this workCorrespondence: Shih-Chun YangDepartment of Cosmetic Science, Providence University, 200 Taiwan Boulevard Sec. 7, Shalu, Taichung 433, TaiwanTel +886-4-26328001 Ext 15041Fax +886-4-26322293Email yangsc@pu.edu.twBackground: It is important to explore the interaction between antibacterial nanoparticles and microbes for understanding bactericidal activity and developing novel applications. It is possible that the nanoparticulate size can govern the antibacterial potency.Purpose: The purpose of this study was to evaluate the antimicrobial and antibiofilm properties of cetylpyridinium chloride (CPC)–decorated nanoemulsions against methicillin-resistant Staphylococcus aureus (MRSA).Methods: The droplet size could be adjusted by varying the percentage of squalene, the main ingredient of the oily core.Results: We fabricated cationic nanoemulsions of three different sizes, 55, 165, and 245 nm. The nanoemulsions showed greater storage stability than the self-assembled CPC micelles. The tested nanoemulsions exhibited more antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria and fungi. The killing of MRSA was mainly induced by direct cell-membrane damage. This rupture led to the leakage of cytoplasmic DNA and proteins. The nanoemulsions might also degrade the DNA helix and disturb protein synthesis. The proteomic analysis indicated the significant downregulation of DNA-directed RNA polymerase (RNAP) subunits β and β’. The antibacterial effect of nanoemulsions increased with decreasing droplet size in the biofilm MRSA but not planktonic MRSA. The small-sized nanoemulsions had potent antibiofilm activity that showed a colony-forming unit (CFU) reduction of 10-fold compared with the control. The loss of total DNA concentration also negatively correlated with the nanoemulsion size.Conclusion: The present report established a foundation for the development of squalene@CPC nanosystems against drug-resistant S. aureus.Keywords: cetylpyridinium chloride, nanoemulsion, MRSA, biofilm, size effect
- Published
- 2019
8. The Cleaning Effect of the Photocatalysis of TiO
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Yan, Gao, Xi, Lin, Yadong, Zhao, Shulan, Xu, Chunhua, Lai, Zehong, Guo, Wangxi, Wu, Xianglong, Ding, Fang, Jia, Lei, Zhou, and Ying, Liu
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Titanium ,nanowires ,Nanowires ,Osseointegration ,Surface Properties ,TiO2 ,osseointegration ,Prostheses and Implants ,titanium implant ,photocatalysis ,Original Research - Abstract
Background Improvements in the early osseointegration of titanium implants require investigations on the bone-implant interface, which is a critical and complex challenge. The surface cleanliness of titanium implants plays an important role at this interface. However, the implant surface would inevitably absorb contamination such as organic hydrocarbons, which is not conductive to the establishment of early osseointegration. Herein, an optimized approach for removing contamination from titanium surfaces was studied. Methods The TiO2-B@anatase NWs (nanowires) were prepared on titanium substrates through a hydrothermal process. A methylene blue degradation experiment was performed to assess the photodegradation activity. The cleaning effect of the photocatalysis of TiO2-B@anatase NWs on a titanium surface and the cellular early response was determined by analyzing cell morphology, attachment, proliferation and differentiation. Results The results indicated that the photocatalysis of TiO2-B@anatase NWs could effectively remove hydrocarbons on titanium surfaces without sacrificing the favourable titanium surface morphology. The methylene blue degradation experiment revealed that the photocatalysis of TiO2-B@anatase NWs had powerful degradation activity, which is attributed to the presence of strong oxidants such as ·OH. In addition, compared to the merely ultraviolet-treated titanium surfaces, the titanium surfaces treated after the NWs photocatalytic cleaning process markedly enhanced cellular early response. Conclusion The photocatalysis of TiO2-B@anatase NWs for the removal of contamination from titanium surfaces has the potential to enable the rapid and complete establishment of early osseointegration.
- Published
- 2020
9. Squarticles as the nanoantidotes to sequester the overdosed antidepressant for detoxification [Corrigendum]
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Chen,Chun-Han, Huang,Tse-Hung, Elzoghby,Ahmed, Wang,Pei-Wen, Chang,Chia-Wen, and Fang,Jia-You
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International Journal of Nanomedicine - Abstract
Chen CH, Huang TH, Elzoghby AO et al. Int JNanomedicine. 2017;12: 8071–8083.The authors missed to mention a grant in theAcknowledgments section of the paper.On page 8082, the Acknowledgments should read asfollows:AcknowledgmentsThe authors are grateful to the Ministry of Scienceand Technology of Taiwan (MOST-105-2320-B-182-010-MY3) and Chang Gung Memorial Hospital(CMRPD1F0231–3 and CMRPG6G0221) for the financialsupport.Read the original article 
- Published
- 2019
10. Oleic acid-loaded nanostructured lipid carrier inhibit neutrophil activities in the presence of albumin and alleviates skin inflammation
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Chen,Chun-Yu, Lee,Ying-Hsuan, Chang,Shih-Hsin, Tsai,Yung-Fong, Fang,Jia-You, and Hwang,Tsong-Long
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Adult ,Male ,Neutrophils ,Administration, Topical ,nanostructured lipid carrier ,Leukotriene B4 ,Neutrophil Activation ,Young Adult ,International Journal of Nanomedicine ,Superoxides ,elastase ,Animals ,Humans ,Original Research ,Skin ,reactive oxygen species ,Inflammation ,Drug Carriers ,Mice, Inbred BALB C ,Cell Death ,Pancreatic Elastase ,neutrophil ,Serum Albumin, Bovine ,Lipids ,Nanostructures ,Calcium ,Cattle ,superoxide ,Oleic Acid - Abstract
Chun-Yu Chen,1,2 Ying-Hsuan Lee,1 Shih-Hsin Chang,1,3–5 Yung-Fong Tsai,1,2 Jia-You Fang,1–3,5 Tsong-Long Hwang1–3,5,61Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; 2Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan; 3Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan; 4Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; 5Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan; 6Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, TaiwanAim: This paper reports on the incorporation of oleic acid (OA) within nanostructured lipid carriers (OA-NLC) to improve the anti-inflammatory effects in the presence of albumin.Materials and methods: NLCs produced via hot high-shear homogenization/ultrasonication were characterized in terms of particle size, zeta potential, and toxicity. We examined the effects of OA-NLC on neutrophil activities. Dermatologic therapeutic potential was also elucidated by using a murine model of leukotriene B4-induced skin inflammation.Results: In the presence of albumin, OA-NLC but not free OA inhibited superoxide generation and elastase release. Topical administration of OA-NLC alleviated neutrophil infiltration and severity of skin inflammation.Conclusion: OA incorporated within NLC can overcome the interference of albumin, which would undermine the anti-inflammatory effects of OA. OA-NLC has potential therapeutic effects in topical ointments.Keywords: oleic acid, nanostructured lipid carrier, neutrophil, reactive oxygen species, superoxide, elastase
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- 2019
11. Liquid crystalline assembly for potential combinatorial chemo–herbal drug delivery to lung cancer cells
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Abdelaziz, Hadeer M., primary, Elzoghby, Ahmed O., additional, Helmy, Maged W., additional, Samaha, Magda W., additional, Fang, Jia-You, additional, and Freag, May S., additional
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- 2019
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12. Anti-MRSA malleable liposomes carrying chloramphenicol for ameliorating hair follicle targeting
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Hsu, Ching-Yun, primary, Yang, Shih-Chun, additional, Sung, Calvin T, additional, Weng, Yi-Han, additional, and Fang, Jia-You, additional
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- 2017
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13. Squarticles as the nanoantidotes to sequester the overdosed antidepressant for detoxification
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Chen, Chun-Han, primary, Huang, Tse-Hung, additional, Elzoghby, Ahmed, additional, Wang, Pei-Wen, additional, Chang, Chia-Wen, additional, and Fang, Jia-You, additional
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- 2017
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14. The effects of hierarchical micro/nanosurfaces decorated with TiO2 nanotubes on the bioactivity of titanium implants in vitro and in vivo
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Lei Zhou, Mingdeng Rong, Xi Lin, Shaobing Li, Jingxu Wang, Qingxia Zhao, Chunhua Lai, Fang Jia, Zehong Guo, Xianglong Ding, Yan Gao, Jingyi Wu, and Haibin Lu
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Male ,Materials science ,dental and orthopedic implant ,Surface Properties ,Biophysics ,Pharmaceutical Science ,chemistry.chemical_element ,Fluorescent Antibody Technique ,Bioengineering ,Nanotechnology ,Osseointegration ,Biomaterials ,Dogs ,In vivo ,International Journal of Nanomedicine ,Cell Line, Tumor ,Drug Discovery ,Cell Adhesion ,Animals ,Humans ,Nanotopography ,RNA, Messenger ,Cell adhesion ,Cell Shape ,Cell Proliferation ,Original Research ,Titanium ,Nanotubes ,Acid etching ,Anodizing ,Organic Chemistry ,fungi ,nanotopography ,Water ,osseointegration ,General Medicine ,Prostheses and Implants ,In vitro ,chemistry ,Chemical engineering ,Gene Expression Regulation ,Adsorption - Abstract
Xianglong Ding,1 Lei Zhou,1 Jingxu Wang,2 Qingxia Zhao,3 Xi Lin,1 Yan Gao,1 Shaobing Li,4 Jingyi Wu,1 Mingdeng Rong,4 Zehong Guo,1 Chunhua Lai,1 Haibin Lu,4 Fang Jia11Center of Oral Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, 2Department of Stomatology, The First Affiliated Hospital of Guangzhou Medical University, 3Department of Stomatology, Nanfang Hospital, 4Department of Periodontics and Implantology, Guangdong Provincial Stomatological Hospital, Southern Medical University, Guangzhou, People’s Republic of ChinaAbstract: In the present work, a hierarchical hybrid micro/nanostructured titanium surface was obtained by sandblasting with large grit and acid etching (SLA), and nanotubes of different diameters (30nm, 50nm, and 80nm) were superimposed by anodization. The effect of each SLA-treated surface decorated with nanotubes (SLA+30nm, SLA+50nm, and SLA+80nm) on osteogenesis was studied in vitro and in vivo. The human MG63 osteosarcoma cell line was used for cytocompatibility evaluation, which showed that cell adhesion and proliferation were dramatically enhanced on SLA+30nm. In comparison with cells grown on the other tested surfaces, those grown on SLA+80nm showed an enhanced expression of osteogenesis-related genes. Cell spread was also enhanced on SLA+80nm. A canine model was used for in vivo evaluation of bone bonding. Histological examination demonstrated that new bone was formed more rapidly on SLA-treated surfaces with nanotubes (especially SLA+80nm) than on those without nanotubes. All of these results indicate that SLA+80nm is favorable for promoting the activity of osteoblasts and early bone bonding.Keywords: nanotopography, osseointegration, dental and orthopedic implant, titanium
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- 2015
15. Liquid crystalline assembly for potential combinatorial chemo-herbal drug delivery to lung cancer cells.
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Abdelaziz, Hadeer M, Elzoghby, Ahmed O, Helmy, Maged W, Samaha, Magda W, Fang, Jia-You, and Freag, May S
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- 2019
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16. Eupafolin nanoparticles protect HaCaT keratinocytes from particulate matter-induced inflammation and oxidative stress
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Yen, Feng-Lin, primary, Lin, Zih-Chan, additional, Lee, Chiang-Wen, additional, Tsai, Ming-Horng, additional, Ko, Horng-Huey, additional, Fang, Jia-You, additional, Chiang, Yao-Chang, additional, Liang, Chan-Jung, additional, Hsu, Lee-Fen Hsu, additional, and Hu, Stephen Chu-Sung, additional
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- 2016
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17. Self-nanoemulsifying drug delivery systems ameliorate the oral delivery of silymarin in rats with Roux-en-Y gastric bypass surgery
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Fang, Jia-You, primary, Chen, Chun-Han, additional, Chang, Cheng-Chih, additional, Shih, Tsung-Hsien, additional, Aljuffali, Ibrahim A., additional, and Yeh, Ta-Sen, additional
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- 2015
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18. Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles
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Fang, Jia-You, primary, Hwang, Tsong-Long, additional, Aljuffali, Ibrahim A., additional, Lin, Chwan-Fwu, additional, and Chang, Cheng-Chih, additional
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- 2015
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19. The effects of hierarchical micro/nanosurfaces decorated with TiO2 nanotubes on the bioactivity of titanium implants in vitro and in vivo.
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Xianglong Ding, Lei Zhou, Jingxu Wang, Qingxia Zhao, Xi Lin, Yan Gao, Shaobing Li, Jingyi Wu, Mingdeng Rong, Zehong Guo, Chunhua Lai, Haibin Lu, and Fang Jia
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- 2015
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20. Combination of calcipotriol and methotrexate in nanostructured lipid carriers for topical delivery
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Fang, Jia-You, primary
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- 2010
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21. A TA/Cu 2+ Nanoparticle Enhanced Carboxymethyl Chitosan-Based Hydrogel Dressing with Antioxidant Properties and Promoting Wound Healing.
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Huang Y, Chen Y, Cheng G, Li W, Zhang H, Yu C, Fang J, Zuo J, Li Y, Xu L, and Sun D
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- Humans, Hydrogels pharmacology, Antioxidants pharmacology, Copper pharmacology, Bandages, Cicatrix, Anti-Bacterial Agents pharmacology, Chitosan, Nanoparticles, Polyphenols
- Abstract
Background: As the first line of immune defense and the largest organ of body, skin is vulnerable to damage caused by surgery, burns, collisions and other factors. Wound healing in the skin is a long and complex physiological process that is influenced by a number of different factors. Proper wound care can greatly improve the speed of wound healing and reduce the generation of scars. However, traditional wound dressings (bandages, gauze, etc.) often used in clinical practice have a single function, lack of active ingredients and are limited in use. Hydrogels with three-dimensional network structure are a potential biomedical material because of their physical and chemical environment similar to extracellular matrix. In particular, hydrogel dressings with low price, good biocompatibility, degradability, antibacterial and angiogenic activity are favored by the public., Methods: Here, a carboxymethyl chitosan-based hydrogel dressing (CMCS-TA/Cu
2+ ) reinforced by copper ion crosslinked tannic acid (TA/Cu2+ ) nanoparticles was developed. This study investigated the physical and chemical characteristics, cytotoxicity, and angiogenesis of TA/Cu2+ nanoparticles and CMCS-TA/Cu2+ hydrogels. Furthermore, a full-thickness skin defect wound model was employed to assess the in vivo wound healing capacity of hydrogel dressings., Results: The introduction of TA/Cu2+ nanoparticles not only could increase the mechanical properties of the hydrogel but also continuously releases copper ions to promote cell migration (the cell migration could reach 92% at 48 h) and tubule formation, remove free radicals and promote wound healing (repair rate could reach 90% at 9 days)., Conclusion: Experiments have proved that CMCS-TA/Cu2+ hydrogel has good cytocompatibility, antioxidant and wound healing ability, providing an advantageous solution for skin repair., Competing Interests: The authors declare that they have no potential conflicts of interest regarding the research, authorship, and/or publication of this article., (© 2024 Huang et al.)- Published
- 2024
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22. Eupafolin nanoparticles protect HaCaT keratinocytes from particulate matter-induced inflammation and oxidative stress.
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Lin ZC, Lee CW, Tsai MH, Ko HH, Fang JY, Chiang YC, Liang CJ, Hsu LF, Hu SC, and Yen FL
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- Animals, Cell Line, Cell Survival drug effects, Crystallization, Cyclooxygenase 2 metabolism, Dinoprostone biosynthesis, Down-Regulation drug effects, Drug Delivery Systems, Excipients, Humans, Inflammation metabolism, Keratinocytes drug effects, Keratinocytes enzymology, Mitogen-Activated Protein Kinases metabolism, NADPH Oxidases metabolism, NF-kappa B metabolism, Particle Size, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Skin drug effects, Skin metabolism, Skin Absorption drug effects, Solubility, Sus scrofa, Cytoprotection drug effects, Flavones pharmacology, Inflammation pathology, Keratinocytes pathology, Nanoparticles chemistry, Oxidative Stress drug effects, Particulate Matter toxicity
- Abstract
Exposure to particulate matter (PM), a major form of air pollution, can induce oxidative stress and inflammation and may lead to many diseases in various organ systems including the skin. Eupafolin, a flavonoid compound derived from Phyla nodiflora, has been previously shown to exhibit various pharmacological activities, including antioxidant and anti-inflammatory effects. Unfortunately, eupafolin is characterized by poor water solubility and skin penetration, which limits its clinical applications. To address these issues, we successfully synthesized a eupafolin nanoparticle delivery system (ENDS). Our findings showed that ENDS could overcome the physicochemical drawbacks of raw eupafolin with respect to water solubility and skin penetration, through reduction of particle size and formation of an amorphous state with hydrogen bonding. Moreover, ENDS was superior to raw eupafolin in attenuating PM-induced oxidative stress and inflammation in HaCaT keratinocytes, by mediating the antioxidant pathway (decreased reactive oxygen species production and nicotinamide adenine dinucleotide phosphate oxidase activity) and anti-inflammation pathway (decreased cyclooxygenase-2 expression and prostaglandin E2 production through downregulation of mitogen-activated protein kinase and nuclear factor-κB signaling). In summary, ENDS shows better antioxidant and anti-inflammatory activities than raw eupafolin through improvement of water solubility and skin penetration. Therefore, ENDS may potentially be used as a medicinal drug and/or cosmeceutical product to prevent PM-induced skin inflammation.
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- 2016
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23. Self-nanoemulsifying drug delivery systems ameliorate the oral delivery of silymarin in rats with Roux-en-Y gastric bypass surgery.
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Chen CH, Chang CC, Shih TH, Aljuffali IA, Yeh TS, and Fang JY
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- Administration, Oral, Animals, Rats, Rats, Sprague-Dawley, Silybin, Solubility, Drug Delivery Systems methods, Emulsions administration & dosage, Emulsions chemistry, Emulsions pharmacokinetics, Gastric Bypass, Nanoparticles administration & dosage, Nanoparticles chemistry, Silymarin administration & dosage, Silymarin blood, Silymarin chemistry, Silymarin pharmacokinetics
- Abstract
Roux-en-Y gastric bypass (RYGB) is a popular surgery to reduce the body weight of obese patients. Although food intake is restricted by RYGB, drug absorption is also decreased. The purpose of this study was to develop novel self-nanoemulsifying drug delivery systems (SNEDDS) for enhancing the oral delivery of silymarin, which has poor water solubility. The SNEDDS were characterized by size, zeta potential, droplet number, and morphology. A technique of RYGB was performed in Sprague-Dawley rats. SNEDDS were administered at a silymarin dose of 600 mg/kg in normal and RYGB rats for comparison with silymarin aqueous suspension and polyethylene glycol (PEG) 400 solution. Plasma silibinin, the main active ingredient in silymarin, was chosen for estimating the pharmacokinetic parameters. SNEDDS diluted in simulated gastric fluid exhibited a droplet size of 190 nm with a spherical shape. The nanocarriers promoted silibinin availability via oral ingestion in RYGB rats by 2.5-fold and 1.5-fold compared to the suspension and PEG 400 solution, respectively. A significant double-peak concentration of silibinin was detected for RYGB rats receiving SNEDDS. Fluorescence imaging showed a deeper and broader penetration of Nile red, the fluorescence dye, into the gastrointestinal mucosa from SNEDDS than from PEG 400 solution. Histological examination showed that SNEDDS caused more minor inflammation at the gastrointestinal membrane as compared with that caused by PEG 400 solution, indicating a shielding of direct silymarin contact with the mucosa by the nanodroplets. SNEDDS generally showed low-level or negligible irritation in the gastrointestinal tract. Silymarin-loaded SNEDDS were successfully developed to improve the dissolution, permeability, and oral bioavailability of silymarin. To the best of our knowledge, this is the first investigation reporting the usefulness of SNEDDS for improving drug malabsorption elicited by gastric bypass surgery.
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- 2015
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24. Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles.
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Hwang TL, Aljuffali IA, Lin CF, Chang YT, and Fang JY
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- Cations, Cell Survival drug effects, Cells, Cultured, Cetrimonium, Cetrimonium Compounds chemistry, Cetrimonium Compounds toxicity, Humans, Particle Size, Lipids chemistry, Lipids toxicity, Nanoparticles chemistry, Nanoparticles toxicity, Neutrophils drug effects, Polymers chemistry, Polymers toxicity
- Abstract
This report compares the effect of lipid and polymeric nanoparticles upon human neutrophils in the presence of cationic surfactants. Nanostructured lipid carriers and poly(lactic-co-glycolic) acid nanoparticles were manufactured as lipid and polymeric systems, respectively. Some cytotoxic and proinflammatory mediators such as lactate dehydrogenase (LDH), elastase, O2(•-), and intracellular Ca(2+) were examined. The nanoparticles showed a size of 170-225 nm. Incorporation of cetyltrimethylammonium bromide or soyaethyl morpholinium ethosulfate, the cationic surfactant, converted zeta potential from a negative to a positive charge. Nanoparticles without cationic surfactants revealed a negligible change on immune and inflammatory responses. Cationic surfactants in both nanoparticulate and free forms induced cell death and the release of mediators. Lipid nanoparticles generally demonstrated a greater response compared to polymeric nanoparticles. The neutrophil morphology observed by electron microscopy confirmed this trend. Cetyltrimethylammonium bromide as the coating material showed more significant activation of neutrophils than soyaethyl morpholinium ethosulfate. Confocal microscope imaging displayed a limited internalization of nanoparticles into neutrophils. It is proposed that cationic nanoparticles interact with the cell membrane, triggering membrane disruption and the following Ca(2+) influx. The elevation of intracellular Ca(2+) induces degranulation and oxidative stress. The consequence of these effects is cytotoxicity and cell death. Caution should be taken when selecting feasible nanoparticulate formulations and cationic additives for consideration of applicability and toxicity.
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- 2015
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25. Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging.
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Wen CJ, Zhang LW, Al-Suwayeh SA, Yen TC, and Fang JY
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- Animals, Cell Line, Drug Carriers chemistry, Endocytosis, Female, Mice, Mice, Nude, Microscopy, Fluorescence, Nanomedicine, Nanoparticles ultrastructure, Particle Size, Apomorphine, Brain metabolism, Liposomes chemistry, Neuroimaging methods, Quantum Dots
- Abstract
Quantum dots (QDs) and apomorphine were incorporated into liposomes to eliminate uptake by the liver and enhance brain targeting. We describe the preparation, physicochemical characterization, in vivo bioimaging, and brain endothelial cell uptake of the theranostic liposomes. QDs and the drug were mainly located in the bilayer membrane and inner core of the liposomes, respectively. Spherical vesicles with a mean diameter of ~140 nm were formed. QDs were completely encapsulated by the vesicles. Nearly 80% encapsulation percentage was achieved for apomorphine. A greater fluorescence intensity was observed in mouse brains treated with liposomes compared to free QDs. This result was further confirmed by ex vivo imaging of the organs. QD uptake by the heart and liver was reduced by liposomal incorporation. Apomorphine accumulation in the brain increased by 2.4-fold after this incorporation. According to a hyperspectral imaging analysis, multifunctional liposomes but not the aqueous solution carried QDs into the brain. Liposomes were observed to have been efficiently endocytosed into bEND3 cells. The mechanisms involved in the cellular uptake were clathrin- and caveola-mediated endocytosis, which were energy-dependent. To the best of our knowledge, our group is the first to develop liposomes with a QD-drug hybrid for the aim of imaging and treating brain disorders.
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- 2012
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26. Oil components modulate the skin delivery of 5-aminolevulinic acid and its ester prodrug from oil-in-water and water-in-oil nanoemulsions.
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Zhang LW, Al-Suwayeh SA, Hung CF, Chen CC, and Fang JY
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- Administration, Topical, Aminolevulinic Acid analogs & derivatives, Analysis of Variance, Animals, Emulsions chemistry, Emulsions toxicity, Erythema chemically induced, Female, Germ-Free Life, Hydrogen-Ion Concentration, Mice, Mice, Nude, Microscopy, Confocal, Nanoparticles chemistry, Nanoparticles toxicity, Particle Size, Permeability, Skin Irritancy Tests, Soybean Oil administration & dosage, Soybean Oil toxicity, Squalene administration & dosage, Squalene toxicity, Swine, Aminolevulinic Acid administration & dosage, Aminolevulinic Acid chemistry, Drug Delivery Systems methods, Soybean Oil chemistry, Squalene chemistry
- Abstract
The study evaluated the potential of nanoemulsions for the topical delivery of 5-aminolevulinic acid (ALA) and methyl ALA (mALA). The drugs were incorporated in oil-in-water (O/W) and water-in-oil (W/O) formulations obtained by using soybean oil or squalene as the oil phase. The droplet size, zeta potential, and environmental polarity of the nanocarriers were assessed as physicochemical properties. The O/W and W/O emulsions showed diameters of 216-256 and 18-125 nm, which, respectively, were within the range of submicron- and nano-sized dispersions. In vitro diffusion experiments using Franz-type cells and porcine skin were performed. Nude mice were used, and skin fluorescence derived from protoporphyrin IX was documented by confocal laser scanning microscopy (CLSM). The loading of ALA or mALA into the emulsions resulted in slower release across cellulose membranes. The release rate and skin flux of topical drug application were adjusted by changing the type of nanocarrier, the soybean oil O/W systems showing the highest skin permeation. This formulation increased ALA flux via porcine skin to 180 nmol/cm(2)/h, which was 2.6-fold that of the aqueous control. The CLSM results showed that soybean oil systems promoted mALA permeation to deeper layers of the skin from ∼100 μm to ∼140 μm, which would be beneficial for treating subepidermal and subcutaneous lesions. Drug permeation from W/O systems did not surpass that from the aqueous solution. An in vivo dermal irritation test indicated that the emulsions were safe for topical administration of ALA and mALA.
- Published
- 2011
- Full Text
- View/download PDF
27. Combination of calcipotriol and methotrexate in nanostructured lipid carriers for topical delivery.
- Author
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Lin YK, Huang ZR, Zhuo RZ, and Fang JY
- Subjects
- Administration, Topical, Animals, Calcitriol administration & dosage, Calcitriol chemistry, Calcitriol pharmacokinetics, Dermatologic Agents administration & dosage, Dermatologic Agents chemistry, Dermatologic Agents pharmacokinetics, Drug Combinations, Drug Compounding methods, Female, Methotrexate administration & dosage, Mice, Mice, Nude, Nanocapsules administration & dosage, Nanocapsules ultrastructure, Particle Size, Calcitriol analogs & derivatives, Lipids chemistry, Methotrexate chemistry, Methotrexate pharmacokinetics, Nanocapsules chemistry, Skin Absorption physiology
- Abstract
The combination of calcipotriol with methotrexate can strengthen the topical therapy for psoriasis. The aim of the present study was to evaluate the potential of nanostructured lipid carriers (NLCs) loaded with lipophilic calcipotriol and hydrophilic methotrexate as topical therapy. NLCs composed of Precirol ATO 5 with various amounts of squalene as the liquid lipid were prepared. The particle size, surface charge, molecular environment, drug permeation, and skin irritation of the carriers were assessed. Hyperproliferative skin was also used as a permeation barrier in this study. It was found that variations in the Precirol/squalene ratio had profound effects on the physicochemical characteristics of the NLCs. The range of particle size of the NLC preparations was 270 to 320 nm, with vehicles containing a higher Precirol amount exhibiting a larger diameter. NLCs with a higher Precirol/squalene ratio also showed greater polarity in their molecular environment. Calcipotriol-loaded NLC systems provided drug fluxes of 0.62 to 1.08 microg/cm(2)/h, which were slightly higher or comparable to the 30% ethanol vehicle (control, 0.72 microg/cm(2)/h). The methotrexate amount permeating the skin was 2.4 to 4.4-times greater using NLCs compared to that with the control. Dual drug-loaded NLCs exhibited reduced skin permeation of calcipotriol but not methotrexate. The in vivo topical delivery examined by confocal laser scanning microscopy (CLSM) showed a good correlation with the in vitro results. These two drugs with extremely different polarities can successfully be combined in NLCs. Results suggest that NLCs may have the potential to serve as delivery carriers for antipsoriatic drugs because of enhanced drug permeation and limited skin irritation.
- Published
- 2010
- Full Text
- View/download PDF
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