1. Development of self-assembled polygalacturonic acid-peptide composites and their interactions with mesenchymal stem cells for potential applications in tendon tissue engineering
- Author
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Harrison T. Pajovich, Steven M. Romanelli, Ipsita A. Banerjee, and Grant A. Knoll
- Subjects
Scaffold ,Dipeptide ,Biocompatibility ,biology ,Chemistry ,Mesenchymal stem cell ,Biomedical Engineering ,Atomic and Molecular Physics, and Optics ,Extracellular matrix ,Biomaterials ,chemistry.chemical_compound ,Tissue engineering ,Biophysics ,biology.protein ,Physical and Theoretical Chemistry ,Elastin ,Type I collagen - Abstract
We have developed a new biomimetic scaffold for potential applications in tendon tissue engineering (TE). The scaffold template was synthesised by conjugating polygalacturonic acid with the dipeptide leucyl-leucine to mimic the leucine rich proteoglycans found in the extracellular matrix (ECM) of tenocytes. To the template, type I collagen and an elastin derived peptide were incorporated in order to form the final PG-Leu-Leu-Col-El scaffold. Results indicated the formation of gelatinous, fibrous scaffolds. DSC analysis showed phase changes that included crystallisation and thermal melting due to re-organisation of the scaffold components. Young's modulus was determined to be 832 ± 2 MPa. Rheology studies showed that the scaffold maintained a constant G´ / GĖ ratio over a wide range of angular frequency. Cell studies with bone marrow derived mesenchymal stem cells (BMSC) indicated that the scaffolds promoted cell proliferation and formed three dimensional cell-scaffold matrices. This newly developed scaffold may open new opportunities for tissue engineering applications.
- Published
- 2019
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