1. Sequential Release of Paclitaxel and Imatinib from Core–Shell Microparticles Prepared by Coaxial Electrospray for Vaginal Therapy of Cervical Cancer
- Author
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Yijun Li, Haini Chen, Xuexin Xue, Zhepeng Liu, Gang Wei, Jun Wang, Fengmei Lv, Shoujin Zhao, Yu Liu, and Weiyue Lu
- Subjects
Combination therapy ,cervical cancer ,QH301-705.5 ,Sodium hyaluronate ,Uterine Cervical Neoplasms ,Apoptosis ,sequential release ,Pharmacology ,Article ,Catalysis ,Inorganic Chemistry ,Mice ,chemistry.chemical_compound ,paclitaxel ,Drug Delivery Systems ,In vivo ,Antineoplastic Combined Chemotherapy Protocols ,Tumor Cells, Cultured ,Animals ,Humans ,Distribution (pharmacology) ,Physical and Theoretical Chemistry ,Biology (General) ,Cytotoxicity ,Molecular Biology ,QD1-999 ,Spectroscopy ,core–shell microparticles ,Cell Proliferation ,Drug Carriers ,Organic Chemistry ,General Medicine ,Xenograft Model Antitumor Assays ,Microspheres ,Computer Science Applications ,Drug Liberation ,PLGA ,Chemistry ,Paclitaxel ,chemistry ,imatinib ,Imatinib Mesylate ,Female ,Intravaginal administration ,coaxial electrospray - Abstract
To optimize the anti-tumor efficacy of combination therapy with paclitaxel (PTX) and imatinib (IMN), we used coaxial electrospray to prepare sequential-release core–shell microparticles composed of a PTX-loaded sodium hyaluronate outer layer and an IMN-loaded PLGA core. The morphology, size distribution, drug loading, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), in vitro release, PLGA degradation, cellular growth inhibition, in vivo vaginal retention, anti-tumor efficacy, and local irritation in a murine orthotopic cervicovaginal tumor model after vaginal administration were characterized. The results show that such core–shell microparticles were of spherical appearance, with an average size of 14.65 μm and a significant drug-loading ratio (2.36% for PTX, 19.5% for IMN, w/w), which might benefit cytotoxicity against cervical-cancer-related TC-1 cells. The DSC curves indicate changes in the phase state of PTX and IMN after encapsulation in microparticles. The FTIR spectra show that drug and excipients are compatible with each other. The release profiles show sequential characteristics in that PTX was almost completely released in 1 h and IMN was continuously released for 7 days. These core–shell microparticles showed synergistic inhibition in the growth of TC-1 cells. Such microparticles exhibited prolonged intravaginal residence, a >, 90% tumor inhibitory rate, and minimal mucosal irritation after intravaginal administration. All results suggest that such microparticles potentially provide a non-invasive local chemotherapeutic delivery system for the treatment of cervical cancer by the sequential release of PTX and IMN. more...
- Published
- 2021