1. Hierarchical ZIF-8 toward Immobilizing
- Author
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Miaad, Adnan, Kai, Li, Jianhua, Wang, Li, Xu, and Yunjun, Yan
- Subjects
Esterification ,Burkholderia cepacia lipase (BCL) ,Cetrimonium ,surfactant ,adsorption method ,Lipase ,Burkholderia cepacia ,biodiesel production ,Enzymes, Immobilized ,Catalysis ,Article ,hierarchical ZIF-8 ,Biofuels ,Cetrimonium Compounds ,Zeolites ,Histidine ,hexahedral ZIF-8 - Abstract
A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent. Burkholderia cepacia lipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.
- Published
- 2018