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Start Over Author "Yang, Yingying" Journal international journal of molecular sciences
6 results on '"Yang, Yingying"'

4. STAT3-Mediated Promoter-Enhancer Interaction Up-Regulates Inhibitor of DNA Binding 1 (ID1) to Promote Colon Cancer Progression.

Author

Lin, Zhike, Liu, Ying, Xu, Tian, Su, Ting, Yang, Yingying, Liang, Runhua, Gu, Songgang, Li, Jie, Song, Xuhong, Liang, Bin, Leng, Zhijun, Li, Yangsihan, Meng, Lele, Luo, Yijing, Chang, Xiaolan, Huang, Dongyang, and Xie, Lingzhu

Subjects
COLON cancer, CANCER invasiveness, GENE enhancers, DNA, COLORECTAL cancer, STAT proteins
Abstract

Background: High expression of inhibitor of DNA binding 1 (ID1) correlates with poor prognosis in colorectal cancer (CRC). Aberrant enhancer activation in regulating ID1 transcription is limited. Methods: Immunohistochemistry (IHC), quantitative RT-PCR (RT-qPCR) and Western blotting (WB) were used to determine the expression of ID1. CRISPR-Cas9 was used to generate ID1 or enhancer E1 knockout cell lines. Dual-luciferase reporter assay, chromosome conformation capture assay and ChIP-qPCR were used to determine the active enhancers of ID1. Cell Counting Kit 8, colony-forming, transwell assays and tumorigenicity in nude mice were used to investigate the biological functions of ID1 and enhancer E1. Results: Human CRC tissues and cell lines expressed a higher level of ID1 than normal controls. ID1 promoted CRC cell proliferation and colony formation. Enhancer E1 actively regulated ID1 promoter activity. Signal transducer and activator of transcription 3 (STAT3) bound to ID1 promoter and enhancer E1 to regulate their activity. The inhibitor of STAT3 Stattic attenuated ID1 promoter and enhancer E1 activity and the expression of ID1. Enhancer E1 knockout down-regulated ID1 expression level and cell proliferation in vitro and in vivo. Conclusions: Enhancer E1 is positively regulated by STAT3 and contributes to the regulation of ID1 to promote CRC cell progression and might be a potential target for anti-CRC drug studies. [ABSTRACT FROM AUTHOR]

Published
2023
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6. Particle Bombardment of the cry2A Gene Cassette Induces Stem Borer Resistance in Sugarcane

Author

Youxiong Que, Jinlong Guo, Yang Yingying, Yachun Su, Chunfeng Wang, Qibin Wu, Shiwu Gao, and Liping Xu

Subjects
0106 biological sciences, 0301 basic medicine, Genetic Vectors, Moths, Genes, Plant, 01 natural sciences, Article, Catalysis, lcsh:Chemistry, cry2A gene, resistance, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Transformation, Genetic, sugarcane, Animals, Transgenes, Cultivar, Plant breeding, Physical and Theoretical Chemistry, Cane, Sugar, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Expression vector, Plant Stems, biology, fungi, Organic Chemistry, food and beverages, stem borer, General Medicine, Plants, Genetically Modified, biology.organism_classification, Saccharum, Computer Science Applications, Horticulture, 030104 developmental biology, Gene cassette, lcsh:Biology (General), lcsh:QD1-999, Glufosinate, chemistry, PEST analysis, particle bombardment, 010606 plant biology & botany
Abstract

Sugarcane borer is the most common and harmful pest in Chinese sugarcane fields, and can cause damage to the whole plant during the entire growing season. To improve borer resistance in sugarcane, we constructed a plant expression vector pGcry2A0229 with the bar gene as the marker and the cry2A gene as the target, and introduced it into embryogenic calli of most widely cultivated sugarcane cultivar ROC22 by particle bombardment. After screening with phosphinothricin in vitro and Basta spray, 21 resistance-regenerated plants were obtained, and 10 positive transgenic lines harboring the cry2A gene were further confirmed by conventional PCR detection. Reverse transcription quantitative PCR (RT-qPCR) analysis showed that the copy number of the cry2A gene varied among different transgenic lines but did not exceed four copies. Quantitative ELISA analysis showed that there was no linear relationship with copy number but negatively correlated with the percentage of borer-infested plants. The analysis of industrial and agronomic traits showed that the theoretical sugar yields of transgenic lines TR-4 and TR-10 were slightly lower than that of the control in both plant cane and ratoon cane, nevertheless, TR-4 and TR-10 lines exhibited markedly lower in frequency of borer-infested plants in plant cane and in the ratoon cane compared to the control. Our results indicate that the introduction of the cry2A gene via bombardment produces transgenic lines with obviously increased stem borer resistance and comparable sugar yield, providing a practical value in direct commercial cultivation and crossbreeding for ROC22 has been used as the most popular elite genitor in various breeding programs in China.

Published
2018

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