7 results on '"Mijowska E"'
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2. The Influence of Graphene Oxide-Fe 3 O 4 Differently Conjugated with 10-Hydroxycampthotecin and a Rotating Magnetic Field on Adenocarcinoma Cells.
- Author
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Jedrzejczak-Silicka M, Szymańska K, Mijowska E, and Rakoczy R
- Subjects
- Humans, Magnetic Fields, Graphite pharmacology, Nanospheres, Adenocarcinoma
- Abstract
Nanoparticles (e.g., graphene oxide, graphene oxide-Fe
3 O4 nanocomposite or hexagonal boron nitride) loaded with anti-cancer drugs and targeted at cancerous cells allowed researchers to determine the most effective in vitro conditions for anticancer treatment. For this reason, the main propose of the present study was to determine the effect of graphene oxide (GO) with iron oxide (Fe3 O4 ) nanoparticles (GO-Fe3 O4 ) covalently (c-GO-Fe3 O4 -HCPT) and non-covalently (nc-GO-Fe3 O4 -HCPT) conjugated with hydroxycamptothecin (HCPT) in the presence of a rotating magnetic field (RMF) on relative cell viability using the MCF-7 breast cancer cell line. The obtained GO-Fe3 O4 nanocomposites demonstrated the uniform coverage of the graphene flakes with the nanospheres, with the thickness of the flakes estimated as ca. 1.2 nm. The XRD pattern of GO-Fe3 O4 indicates that the crystal structure of the magnetite remained stable during the functionalization with HCPT that was confirmed with FTIR spectra. After 24 h, approx. 49% and 34% of the anti-cancer drug was released from nc-GO-Fe3 O4 -HCPT and c-GO-Fe3 O4 -HCPT, respectively. The stronger bonds in the c-GO-Fe3 O4 -HCPT resulted in a slower release of a smaller drug amount from the nanocomposite. The combined impact of the novel nanocomposites and a rotating magnetic field on MCF-7 cells was revealed and the efficiency of this novel approach has been confirmed. However, MCF-7 cells were more significantly affected by nc-GO-Fe3 O4 -HCPT. In the present study, it was found that the concentration of nc-GO-Fe3 O4 -HCPT and a RMF has the highest statistically significant influence on MCF-7 cell viability. The obtained novel nanocomposites and rotating magnetic field were found to affect the MCF-7 cells in a dose-dependent manner. The presented results may have potential clinical applications, but still, more in-depth analyses need to be performed.- Published
- 2024
- Full Text
- View/download PDF
3. Nanoparticles Influence Lytic Phage T4-like Performance In Vitro.
- Author
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Stachurska X, Cendrowski K, Pachnowska K, Piegat A, Mijowska E, and Nawrotek P
- Subjects
- Bacteriophage T4, Nanoparticles, Silicon Dioxide
- Abstract
Little is known about interactions of non-filamentous, complex-structured lytic phages and free, non-ordered nanoparticles. Emerging questions about their possible bio-sanitization co-applications or predictions of possible contact effects in the environment require testing. Therefore, we revealed the influence of various nanoparticles (NPs; SiO2, TiO2-SiO2, TiO2, Fe3O4, Fe3O4-SiO2 and SiO2-Fe3O4-TiO2) on a T4-like phage. In great detail, we investigated phage plaque-forming ability, phage lytic performance, phage progeny burst times and titers by the eclipse phase determinations. Additionally, it was proved that TEM micrographs and results of NP zeta potentials (ZP) were crucial to explain the obtained microbiological data. We propose that the mere presence of the nanoparticle charge is not sufficient for the phage to attach specifically to the NPs, consequently influencing the phage performance. The zeta potential values in the NPs are of the greatest influence. The threshold values were established at ZP < −35 (mV) for phage tail binding, and ZP > 35 (mV) for phage head binding. When NPs do not meet these requirements, phage−nanoparticle physical interaction becomes nonspecific. We also showed that NPs altered the phage lytic activity, regardless of the used NP concentration. Most of the tested nanoparticles positively influenced the phage lytic performance, except for SiO2 and Fe3O4-SiO2, with a ZP lower than −35 (mV), binding with the phage infective part—the tail.
- Published
- 2022
- Full Text
- View/download PDF
4. Influence of Hydrogenation on Morphology, Chemical Structure and Photocatalytic Efficiency of Graphitic Carbon Nitride.
- Author
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Baranowska D, Kędzierski T, Aleksandrzak M, Mijowska E, and Zielińska B
- Subjects
- Catalysis, Hot Temperature, Hydrogenation, Light, Photochemical Processes, Graphite chemistry, Nitrogen Compounds chemistry, Rhodamines chemistry
- Abstract
In this contribution, the effect of hydrogenation conditions atmosphere (temperature and time) on physicochemical properties and photocatalytic efficiency of graphitic carbon nitride (g-C
3 N4 , gCN) was studied in great details. The changes in the morphology, chemical structure, optical and electrochemical properties were carefully investigated. Interestingly, the as-modified samples exhibited boosted photocatalytic degradation of Rhodamine B (RhB) with the assistance of visible light irradiation. Among modified gCN, the sample annealed at 500 °C for 4 h (500-4) in H2 atmosphere exhibited the highest photocatalytic activity-1.76 times higher compared to pristine gCN. Additionally, this sample presented high stability and durability after four cycles. It was noticed that treating gCN with hydrogen at elevated temperatures caused the creation of nitrogen vacancies on gCN surfaces acting as highly active sites enhancing the specific surface area and improving the mobility of photogenerated charge carriers leading to accelerating the photocatalytic activity. Therefore, it is believed that detailed optimization of thermal treatment in a hydrogen atmosphere is a facile approach to boost the photoactivity of gCN.- Published
- 2021
- Full Text
- View/download PDF
5. Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO with Enhanced Antibacterial and Mechanical Properties.
- Author
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Jabłońska J, Onyszko M, Konopacki M, Augustyniak A, Rakoczy R, and Mijowska E
- Subjects
- Anti-Infective Agents, Cellulose ultrastructure, Escherichia coli, Mechanical Tests, Metal Nanoparticles chemistry, Metal Nanoparticles ultrastructure, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Nanocomposites ultrastructure, Nanofibers chemistry, Nanofibers ultrastructure, Surface Properties, Tensile Strength, X-Ray Diffraction, Biopolymers chemistry, Biopolymers pharmacology, Cellulose chemistry, Chitosan chemistry, Nanocomposites chemistry, Product Packaging methods, Zinc Oxide chemistry
- Abstract
Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity. We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles. The proposed processing of bacterial cellulose resulted in the formation of nanofibers. Antimicrobial behavior was tested using E. coli ATCC
® 25922™ following the ASTM E2149-13a standard. The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard. The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties. Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.- Published
- 2021
- Full Text
- View/download PDF
6. Boosting of Antibacterial Performance of Cellulose Based Paper Sheet via TiO 2 Nanoparticles.
- Author
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Maślana K, Żywicka A, Wenelska K, and Mijowska E
- Subjects
- Anti-Infective Agents, Escherichia coli, Microbial Sensitivity Tests, Paper, Reproducibility of Results, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus, Stem Cells, Thermogravimetry, Anti-Bacterial Agents pharmacology, Cellulose chemistry, Materials Testing, Metal Nanoparticles chemistry, Titanium chemistry
- Abstract
Here, we aimed to boost antibacterial performance of cellulose fibers for paper sheet application. Therefore, TiO
2 nanoparticles have been used with controlled loading onto the surface of the fibers. A simple and facile composite preparation route based on ultrasound and mechanical assisted stirring has been developed. We tested cellulose paper enriched by TiO2 from 1.0 wt% to 8.0 wt%, respectively. Antibacterial performance has been studied against Staphylococcus aureus and Escherichia coli bacteria. Studies showed that all composites exhibit significant capability to reduce living cells of S. aureus and E. coli bacteria at least 60%. The simplicity, low cost, and reproducibility of the prepared method indicates the potential to be scaled up for industrial applications.- Published
- 2021
- Full Text
- View/download PDF
7. The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes.
- Author
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Augustyniak A, Cendrowski K, Grygorcewicz B, Jabłońska J, Nawrotek P, Trukawka M, Mijowska E, and Popowska M
- Subjects
- Bacterial Outer Membrane Proteins genetics, Biofilms drug effects, Biofilms growth & development, Drug Resistance, Multiple drug effects, Drug Resistance, Multiple genetics, Gene Expression Regulation, Bacterial drug effects, Humans, Membrane Transport Proteins genetics, Microbial Sensitivity Tests, Microscopy, Electron, Transmission, Nanocomposites radiation effects, Nanocomposites ultrastructure, Nanotubes ultrastructure, Pseudomonas Infections microbiology, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa physiology, Spectrometry, X-Ray Emission, Ultraviolet Rays, X-Ray Diffraction, Nanocomposites administration & dosage, Nanotubes chemistry, Pseudomonas aeruginosa drug effects, Silicon Dioxide chemistry
- Abstract
Pseudomonas aeruginosa is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of P. aeruginosa PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the mexA coding efflux pump was upregulated, which could be associated with an interaction with TiO
2 . The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of P. aeruginosa PAO1.- Published
- 2020
- Full Text
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