1. Multiplatform Investigation of Plasma and Tissue Lipid Signatures of Breast Cancer Using Mass Spectrometry Tools
- Author
-
Livia S. Eberlin, Alessandra Tata, Marcos N. Eberlin, Marcella Regina Cardoso, Fernando Guimarães, Denise Gonçalves Priolli, Michael Murgu, Andreia M Porcari, Luciana Montes Rezende, Sophie Françoise Mauricette Derchain, Alex Ap Rosini Silva, John Q. Lin, and Geisilene Russano de Paiva Silva
- Subjects
0301 basic medicine ,Adult ,Pathology ,medicine.medical_specialty ,Spectrometry, Mass, Electrospray Ionization ,Breast Neoplasms ,Mass spectrometry ,01 natural sciences ,Catalysis ,Article ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,Breast cancer ,breast cancer ,Liquid chromatography–mass spectrometry ,Lipidomics ,medicine ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,liquid chromatography-mass spectrometry ,plasma ,Aged ,Aged, 80 and over ,Desorption electrospray ionization ,Chemistry ,010401 analytical chemistry ,Organic Chemistry ,Cancer ,General Medicine ,Plasma ,Middle Aged ,medicine.disease ,Lipid Metabolism ,Lipids ,0104 chemical sciences ,Computer Science Applications ,Tissue lipid ,Carcinoma, Ductal ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,lipidomics ,Female ,tumor tissue ,desorption-electrospray-ionization—mass spectrometry imaging - Abstract
Plasma and tissue from breast cancer patients are valuable for diagnostic/prognostic purposes and are accessible by multiple mass spectrometry (MS) tools. Liquid chromatography-mass spectrometry (LC-MS) and ambient mass spectrometry imaging (MSI) were shown to be robust and reproducible technologies for breast cancer diagnosis. Here, we investigated whether there is a correspondence between lipid cancer features observed by desorption electrospray ionization (DESI)-MSI in tissue and those detected by LC-MS in plasma samples. The study included 28 tissues and 20 plasma samples from 24 women with ductal breast carcinomas of both special and no special type (NST) along with 22 plasma samples from healthy women. The comparison of plasma and tissue lipid signatures revealed that each one of the studied matrices (i.e., blood or tumor) has its own specific molecular signature and the full interposition of their discriminant ions is not possible. This comparison also revealed that the molecular indicators of tissue injury, characteristic of the breast cancer tissue profile obtained by DESI-MSI, do not persist as cancer discriminators in peripheral blood even though some of them could be found in plasma samples.
- Published
- 2020