1. CD90 Is Dispensable for White and Beige/Brown Adipocyte Differentiation.
- Author
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Dahlhaus M, Roos J, Engel D, Tews D, Halbgebauer D, Funcke JB, Kiener S, Schuler PJ, Döscher J, Hoffmann TK, Zinngrebe J, Rojewski M, Schrezenmeier H, Debatin KM, Wabitsch M, and Fischer-Posovszky P
- Subjects
- Adipose Tissue, Beige metabolism, Adipose Tissue, Brown metabolism, Adipose Tissue, White cytology, Adipose Tissue, White metabolism, Adult, Arrhythmias, Cardiac metabolism, CRISPR-Cas Systems, Cell Differentiation, Cells, Cultured, Female, Flow Cytometry, Gene Knockout Techniques, Genetic Diseases, X-Linked metabolism, Gigantism metabolism, Heart Defects, Congenital metabolism, Humans, Intellectual Disability metabolism, Male, Middle Aged, Stromal Cells metabolism, Thermogenesis, Up-Regulation, Adipose Tissue, Beige cytology, Adipose Tissue, Brown cytology, Arrhythmias, Cardiac genetics, Genetic Diseases, X-Linked genetics, Gigantism genetics, Heart Defects, Congenital genetics, Intellectual Disability genetics, Thy-1 Antigens genetics, Thy-1 Antigens metabolism
- Abstract
Brown adipose tissue (BAT) is a thermogenic organ in rodents and humans. In mice, the transplantation of BAT has been successfully used to combat obesity and its comorbidities. While such beneficial properties of BAT are now evident, the developmental and cellular origins of brown, beige, and white adipocytes have remained only poorly understood, especially in humans. We recently discovered that CD90 is highly expressed in stromal cells isolated from human white adipose tissue (WAT) compared to BAT. Here, we studied whether CD90 interferes with brown or white adipogenesis or white adipocyte beiging. We applied flow cytometric sorting of human adipose tissue stromal cells (ASCs), a CRISPR/Cas9 knockout strategy in the human Simpson-Golabi-Behmel syndrome (SGBS) adipocyte model system, as well as a siRNA approach in human approaches supports the hypothesis that CD90 affects brown or white adipogenesis or white adipocyte beiging in humans. Taken together, our findings call the conclusions drawn from previous studies, which claimed a central role of CD90 in adipocyte differentiation, into question.
- Published
- 2020
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