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Your search keyword '"Guescini Michele"' showing total 6 results
Start Over Author "Guescini Michele" Journal international journal of molecular sciences
6 results on '"Guescini Michele"'

1. Modulation of the Circulating Extracellular Vesicles in Response to Different Exercise Regimens and Study of Their Inflammatory Effects

Author

Maggio, Serena, primary, Canonico, Barbara, additional, Ceccaroli, Paola, additional, Polidori, Emanuela, additional, Cioccoloni, Andrea, additional, Giacomelli, Luca, additional, Ferri Marini, Carlo, additional, Annibalini, Giosuè, additional, Gervasi, Marco, additional, Benelli, Piero, additional, Fabbri, Francesco, additional, Del Coco, Laura, additional, Fanizzi, Francesco Paolo, additional, Giudetti, Anna Maria, additional, Lucertini, Francesco, additional, and Guescini, Michele, additional

Published
2023
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2. Extracellular Vesicles from Campylobacter jejuni CDT-Treated Caco-2 Cells Inhibit Proliferation of Tumour Intestinal Caco-2 Cells and Myeloid U937 Cells: Detailing the Global Cell Response for Potential Application in Anti-Tumour Strategies

Author

Montanari, Mariele, primary, Guescini, Michele, additional, Gundogdu, Ozan, additional, Luchetti, Francesca, additional, Lanuti, Paola, additional, Ciacci, Caterina, additional, Burattini, Sabrina, additional, Campana, Raffaella, additional, Ortolani, Claudio, additional, Papa, Stefano, additional, and Canonico, Barbara, additional

Published
2022
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3. Extracellular Vesicles from Campylobacter jejuni CDT-Treated Caco-2 Cells Inhibit Proliferation of Tumour Intestinal Caco-2 Cells and Myeloid U937 Cells: Detailing the Global Cell Response for Potential Application in Anti-Tumour Strategies.

Author

Montanari, Mariele, Guescini, Michele, Gundogdu, Ozan, Luchetti, Francesca, Lanuti, Paola, Ciacci, Caterina, Burattini, Sabrina, Campana, Raffaella, Ortolani, Claudio, Papa, Stefano, and Canonico, Barbara

Subjects
*MYELOID cells, *CAMPYLOBACTER jejuni, *EXTRACELLULAR vesicles, *CELL cycle, *CELL proliferation, *BACTERIAL toxins, *CELL death
Abstract

Cytolethal distending toxin (CDT) is produced by a range of Gram-negative pathogenic bacteria such as Campylobacter jejuni. CDT represents an important virulence factor that is a heterotrimeric complex composed of CdtA, CdtB, and CdtC. CdtA and CdtC constitute regulatory subunits whilst CdtB acts as the catalytic subunit exhibiting phosphatase and DNase activities, resulting in cell cycle arrest and cell death. Extracellular vesicle (EV) secretion is an evolutionarily conserved process that is present throughout all kingdoms. Mammalian EVs play important roles in regular cell-to-cell communications but can also spread pathogen- and host-derived molecules during infections to alter immune responses. Here, we demonstrate that CDT targets the endo-lysosomal compartment, partially evading lysosomal degradation and exploiting unconventional secretion (EV release), which is largely involved in bacterial infections. CDT-like effects are transferred by Caco-2 cells to uninfected heterologous U937 and homologous Caco-2 cells. The journey of EVs derived from CDT-treated Caco-2 cells is associated with both intestinal and myeloid tumour cells. EV release represents the primary route of CDT dissemination, revealing an active toxin as part of the cargo. We demonstrated that bacterial toxins could represent suitable tools in cancer therapy, highlighting both the benefits and limitations. The global cell response involves a moderate induction of apoptosis and autophagic features may play a protective role against toxin-induced cell death. EVs from CDT-treated Caco-2 cells represent reliable CDT carriers, potentially suitable in colorectal cancer treatments. Our data present a potential bacterial-related biotherapeutic supporting a multidrug anticancer protocol. [ABSTRACT FROM AUTHOR]

Published
2023
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4. Diameters and Fluorescence Calibration for Extracellular Vesicle Analyses by Flow Cytometry

Author

Simeone, Pasquale, primary, Celia, Christian, additional, Bologna, Giuseppina, additional, Ercolino, Eva, additional, Pierdomenico, Laura, additional, Cilurzo, Felisa, additional, Grande, Rossella, additional, Diomede, Francesca, additional, Vespa, Simone, additional, Canonico, Barbara, additional, Guescini, Michele, additional, Stocchi, Vilberto, additional, Lotti, Lavinia Vittoria, additional, Guagnano, Maria Teresa, additional, Stellin, Luisa, additional, Papa, Stefano, additional, Trubiani, Oriana, additional, Marchisio, Marco, additional, Miscia, Sebastiano, additional, and Lanuti, Paola, additional

Published
2020
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