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Your search keyword '"Gogliettino, Marta"' showing total 8 results
Start Over Author "Gogliettino, Marta" Journal international journal of molecular sciences
8 results on '"Gogliettino, Marta"'

8. Oxidized Substrates of APEH as a Tool to Study the Endoprotease Activity of the Enzyme.

Author

Sandomenico, Annamaria, Gogliettino, Marta, Iaccarino, Emanuela, Fusco, Carmela, Caporale, Andrea, Ruvo, Menotti, Palmieri, Gianna, and Cocca, Ennio

Subjects
*ENZYMES, *PROTEOLYSIS, *C-terminal residues, *METHIONINE, *SERINE, *PROTEOLYTIC enzymes
Abstract

APEH is a ubiquitous and cytosolic serine protease belonging to the prolyl oligopeptidase (POP) family, playing a critical role in the processes of degradation of proteins through both exo- and endopeptidase events. Endopeptidase activity has been associated with protein oxidation; however, the actual mechanisms have yet to be elucidated. We show that a synthetic fragment of GDF11 spanning the region 48–64 acquires sensitivity to the endopeptidase activity of APEH only when the methionines are transformed into the corresponding sulphoxide derivatives. The data suggest that the presence of sulphoxide-modified methionines is an important prerequisite for the substrates to be processed by APEH and that the residue is crucial for switching the enzyme activity from exo- to endoprotease. The cleavage occurs on residues placed on the C-terminal side of Met(O), with an efficiency depending on the methionine adjacent residues, which thereby may play a crucial role in driving and modulating APEH endoprotease activity. [ABSTRACT FROM AUTHOR]

Published
2022
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