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Start Over Author "Fini, Milena" Journal international journal of molecular sciences
31 results on '"Fini, Milena"'

3. Magnesium Alloys in Orthopedics: A Systematic Review on Approaches, Coatings and Strategies to Improve Biocompatibility, Osteogenic Properties and Osteointegration Capabilities

Author

Giavaresi, Gianluca, primary, Bellavia, Daniele, additional, De Luca, Angela De, additional, Costa, Viviana, additional, Raimondi, Lavinia, additional, Cordaro, Aurora, additional, Sartori, Maria, additional, Terrando, Silvio, additional, Toscano, Angelo, additional, Pignatti, Giovanni, additional, and Fini, Milena, additional

Published
2023
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6. Human Osteoblasts’ Response to Biomaterials for Subchondral Bone Regeneration in Standard and Aggressive Environments

Author

Pagani, Stefania, primary, Salerno, Manuela, additional, Filardo, Giuseppe, additional, Locs, Janis, additional, van Osch, Gerjo J.V.M., additional, Vecstaudza, Jana, additional, Dolcini, Laura, additional, Borsari, Veronica, additional, Fini, Milena, additional, Giavaresi, Gianluca, additional, and Columbaro, Marta, additional

Published
2023
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8. Magnesium Alloys in Orthopedics: A Systematic Review on Approaches, Coatings and Strategies to Improve Biocompatibility, Osteogenic Properties and Osteointegration Capabilities.

Author

Giavaresi, Gianluca, Bellavia, Daniele, De Luca, Angela, Costa, Viviana, Raimondi, Lavinia, Cordaro, Aurora, Sartori, Maria, Terrando, Silvio, Toscano, Angelo, Pignatti, Giovanni, and Fini, Milena

Subjects
MAGNESIUM alloys, OSSEOINTEGRATION, ORTHOPEDIC apparatus, ORTHOPEDICS, BONE regeneration, SURFACE coatings
Abstract

There is increasing interest in using magnesium (Mg) alloy orthopedic devices because of their mechanical properties and bioresorption potential. Concerns related to their rapid degradation have been issued by developing biodegradable micro- and nanostructured coatings to enhance corrosion resistance and limit the release of hydrogen during degradation. This systematic review based on four databases (PubMed®, Embase, Web of Science™ and ScienceDirect®) aims to present state-of-the-art strategies, approaches and materials used to address the critical factors currently impeding the utilization of Mg alloy devices. Forty studies were selected according to PRISMA guidelines and specific PECO criteria. Risk of bias assessment was conducted using OHAT and SYRCLE tools for in vitro and in vivo studies, respectively. Despite limitations associated with identified bias, the review provides a comprehensive analysis of preclinical in vitro and in vivo studies focused on manufacturing and application of Mg alloys in orthopedics. This attests to the continuous evolution of research related to Mg alloy modifications (e.g., AZ91, LAE442 and WE43) and micro- and nanocoatings (e.g., MAO and MgF2), which are developed to improve the degradation rate required for long-term mechanical resistance to loading and excellent osseointegration with bone tissue, thereby promoting functional bone regeneration. Further research is required to deeply verify the safety and efficacy of Mg alloys. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Bone Regeneration Guided by a Magnetized Scaffold in an Ovine Defect Model

Author

Maglio, Melania, primary, Sartori, Maria, additional, Gambardella, Alessandro, additional, Shelyakova, Tatiana, additional, Dediu, Valentin Alek, additional, Santin, Matteo, additional, Piñeiro, Yolanda, additional, López, Manuel Bañobre, additional, Rivas, Josè, additional, Tampieri, Anna, additional, Sprio, Simone, additional, Martini, Lucia, additional, Gatti, Alessandro, additional, Russo, Alessandro, additional, Giavaresi, Gianluca, additional, and Fini, Milena, additional

Published
2023
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14. Gender-Specific Differences in Human Vertebral Bone Marrow Clot.

Author

Salamanna, Francesca, Contartese, Deyanira, Borsari, Veronica, Pagani, Stefania, Sartori, Maria, Tschon, Matilde, Griffoni, Cristiana, Giavaresi, Gianluca, Tedesco, Giuseppe, Barbanti Brodano, Giovanni, Gasbarrini, Alessandro, and Fini, Milena

Subjects
HOMEOBOX genes, GENE expression, SPINAL fusion, MESENCHYMAL stem cells, CARTILAGE regeneration, CELLULAR therapy
Abstract

Recently, our group described the application of vertebral bone marrow (vBMA) clot as a cell therapy strategy for spinal fusion. Its beneficial effects were confirmed in aging-associated processes, but the influence of gender is unknown. In this study, we compared the biological properties of vBMA clots and derived vertebral mesenchymal stem cells (MSCs) from female and male patients undergoing spinal fusion procedures and treated with vBMA clot. We analyzed the expression of growth factors (GFs) in vBMA clots and MSCs as well as morphology, viability, doubling time, markers expression, clonogenicity, differentiation ability, senescence factors, Klotho expression, and HOX and TALE gene profiles from female and male donors. Our findings indicate that vBMA clots and derived MSCs from males had higher expression of GFs and greater osteogenic and chondrogenic potential compared to female patients. Additionally, vBMA-clot-derived MSCs from female and male donors exhibited distinct levels of HOX and TALE gene expression. Specifically, HOXA1, HOXB8, HOXD9, HOXA11, and PBX1 genes were upregulated in MSCs derived from clotted vBMA from male donors. These results demonstrate that vBMA clots can be effectively used for spinal fusion procedures; however, gender-related differences should be taken into consideration when utilizing vBMA-clot-based studies to optimize the design and implementation of this cell therapy strategy in clinical trials. [ABSTRACT FROM AUTHOR]

Published
2023
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18. Timing Expression of miR203a-3p during OA Disease: Preliminary In Vitro Evidence.

Author

Costa, Viviana, De Fine, Marcello, Raimondi, Lavinia, Bellavia, Daniele, Cordaro, Aurora, Carina, Valeria, Alessandro, Riccardo, Pignatti, Giovanni, Fini, Milena, Giavaresi, Gianluca, and De Luca, Angela

Subjects
GENE expression, OSTEOARTHRITIS, INFLAMMATION, DEGENERATION (Pathology), BONE diseases, MARINE toxins
Abstract

Osteoarthritis (OA) is a degenerative bone disease that involves the microenvironment and macroenvironment of joints. Progressive joint tissue degradation and loss of extracellular matrix elements, together with different grades of inflammation, are important hallmarks of OA disease. Therefore, the identification of specific biomarkers to distinguish the stages of disease becomes a primary necessity in clinical practice. To this aim, we investigated the role of miR203a-3p in OA progression starting from the evidence obtained by osteoblasts isolated from joint tissues of OA patients classified according to different Kellgren and Lawrence (KL) grading (KL ≤ 3 and KL > 3) and hMSCs treated with IL-1β. Through qRT-PCR analysis, it was found that osteoblasts (OBs) derived from the KL ≤ 3 group expressed high levels of miR203a-3p and low levels of ILs compared with those of OBs derived from the KL > 3 group. The stimulation with IL-1β improved the expression of miR203a-3p and the methylation of the IL-6 promoter gene, favoring an increase in relative protein expression. The gain and loss of function studies showed that the transfection with miR203a-3p inhibitor alone or in co-treatments with IL-1β was able to induce the expression of CX-43 and SP-1 and to modulate the expression of TAZ, in OBs derived from OA patients with KL ≤ 3 compared with KL > 3. These events, confirmed also by qRT-PCR analysis, Western blot, and ELISA assay performed on hMSCs stimulated with IL-1β, supported our hypothesis about the role of miR203a-3p in OA progression. The results suggested that during the early stage, miR203a-3p displayed a protective role reducing the inflammatory effects on CX-43, SP-1, and TAZ. During the OA progression the downregulation of miR203a-3p and consequently the upregulation of CX-43/SP-1 and TAZ expression improved the inflammatory response and the reorganization of the cytoskeleton. This role led to the subsequent stage of the disease, where the aberrant inflammatory and fibrotic responses determined the destruction of the joint. [ABSTRACT FROM AUTHOR]

Published
2023
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19. Potential Anti-Metastatic Role of the Novel miR-CT3 in Tumor Angiogenesis and Osteosarcoma Invasion

Author

Raimondi, Lavinia, primary, Gallo, Alessia, additional, Cuscino, Nicola, additional, De Luca, Angela, additional, Costa, Viviana, additional, Carina, Valeria, additional, Bellavia, Daniele, additional, Bulati, Matteo, additional, Alessandro, Riccardo, additional, Fini, Milena, additional, Conaldi, Pier Giulio, additional, and Giavaresi, Gianluca, additional

Published
2022
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21. How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights

Author

Costa, Viviana, primary, De Fine, Marcello, additional, Carina, Valeria, additional, Conigliaro, Alice, additional, Raimondi, Lavinia, additional, De Luca, Angela, additional, Bellavia, Daniele, additional, Salamanna, Francesca, additional, Alessandro, Riccardo, additional, Pignatti, Giovanni, additional, Fini, Milena, additional, and Giavaresi, Gianluca, additional

Published
2021
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24. Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption

Author

Desando, Giovanna, primary, Bartolotti, Isabella, additional, Martini, Lucia, additional, Giavaresi, Gianluca, additional, Nicoli Aldini, Nicolò, additional, Fini, Milena, additional, Roffi, Alice, additional, Perdisa, Francesco, additional, Filardo, Giuseppe, additional, Kon, Elizaveta, additional, and Grigolo, Brunella, additional

Published
2019
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25. A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration

Author

Roffi, Alice, primary, Kon, Elizaveta, additional, Perdisa, Francesco, additional, Fini, Milena, additional, Di Martino, Alessandro, additional, Parrilli, Annapaola, additional, Salamanna, Francesca, additional, Sandri, Monica, additional, Sartori, Maria, additional, Sprio, Simone, additional, Tampieri, Anna, additional, Marcacci, Maurilio, additional, and Filardo, Giuseppe, additional

Published
2019
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28. The Binomial 'Inflammation-Epigenetics' in Breast Cancer Progression and Bone Metastasis: IL-1β Actions Are Influenced by TET Inhibitor in MCF-7 Cell Line

Author

Daniele Bellavia, Viviana Costa, Angela De Luca, Aurora Cordaro, Milena Fini, Gianluca Giavaresi, Fabio Caradonna, Lavinia Raimondi, Bellavia, Daniele, Costa, Viviana, De Luca, Angela, Cordaro, Aurora, Fini, Milena, Giavaresi, Gianluca, Caradonna, Fabio, and Raimondi, Lavinia

Subjects
Epithelial-Mesenchymal Transition, DNA methylation, bone metastasis, inflammation, Interleukin-1β, ten-eleven translocation proteins, MCF-7 cell line, Interleukin-1beta, Breast Neoplasms, Bone Neoplasms, Catalysis, Epigenesis, Genetic, Inorganic Chemistry, Settore BIO/13 - Biologia Applicata, Cell Line, Tumor, Humans, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Organic Chemistry, General Medicine, Computer Science Applications, Settore BIO/18 - Genetica, MCF-7 Cells, Female, Inflammatory Breast Neoplasms
Abstract

The existence of a tight relationship between inflammation and epigenetics that in primary breast tumor cells can lead to tumor progression and the formation of bone metastases was investigated. It was highlighted how the induction of tumor progression and bone metastasis by Interleukin-1 beta, in a non-metastatic breast cancer cell line, MCF-7, was dependent on the de-methylating actions of ten-eleven translocation proteins (TETs). In fact, the inhibition of their activity by the Bobcat339 molecule, an inhibitor of TET enzymes, determined on the one hand, the modulation of the epithelial-mesenchymal transition process, and on the other hand, the reduction in the expression of markers of bone metastasis, indicating that the epigenetic action of TETs is a prerequisite for IL-1β-dependent tumor progression and bone metastasis formation.

Published
2022
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29. How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights

Author

Angela De Luca, Alice Conigliaro, Viviana Costa, Francesca Salamanna, Marcello De Fine, Gianluca Giavaresi, Riccardo Alessandro, Daniele Bellavia, Milena Fini, Valeria Carina, Giovanni Pignatti, Lavinia Raimondi, Costa, Viviana, De Fine, Marcello, Carina, Valeria, Conigliaro, Alice, Raimondi, Lavinia, De Luca, Angela, Bellavia, Daniele, Salamanna, Francesca, Alessandro, Riccardo, Pignatti, Giovanni, Fini, Milena, and Giavaresi, Gianluca

Subjects
Male, 0301 basic medicine, Bone disease, chondrocytes, Osteoarthritis, CX43, lcsh:Chemistry, 0302 clinical medicine, lcsh:QH301-705.5, Cells, Cultured, Spectroscopy, microRNA, osteoblasts, General Medicine, Middle Aged, Prognosis, Computer Science Applications, microRNAs, mir-31, 030220 oncology & carcinogenesis, chondrocyte, osteoblast, Female, medicine.symptom, Signal Transduction, Adult, Sp1 Transcription Factor, Inflammation, Biology, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, medicine, Humans, Physical and Theoretical Chemistry, Bone regeneration, Molecular Biology, Gene, Loss function, Aged, Organic Chemistry, medicine.disease, SP1, osteoarthritis, 030104 developmental biology, Gene Expression Regulation, lcsh:Biology (General), lcsh:QD1-999, Connexin 43, Cancer research, Follow-Up Studies
Abstract

Osteoarthritis (OA) is a degenerative bone disease that involved micro and macro-environment of joints. To date, there are no radical curative treatments for OA and novel therapies are mandatory. Recent evidence suggests the role of miRNAs in OA progression. In our previous studies, we demonstrated the role of miR-31-5p and miR-33a families in different bone regeneration signaling. Here, we investigated the role of miR-31-5p and miR-33a-5p in OA progression. A different expression of miR-31-5p and miR-33a-5p into osteoblasts and chondrocytes isolated from joint tissues of OA patients classified in based on different Kellgren and Lawrence (KL) grading was highlighted, and through a bioinformatic approach the common miRNAs target Specificity proteins (Sp1) were identified. Sp1 regulates the expression of gap junction protein Connexin43 (Cx43), which in OA drives the modification of i) osteoblasts and chondrocytes genes expression, ii) joint inflammation cytokines releases and iii) cell functions. Concerning this, thanks to gain and loss of function studies, the possible role of Sp1 as a modulator of CX43 expression through miR-31-5p and miR-33a-5p action was also evaluated. Finally, we hypothesize that both miRNAs cooperate to modulate the expression of SP1 in osteoblasts and chondrocytes and interfering, consequently, with CX43 expression, and they might be further investigated as new possible biomarkers for OA.

Published
2021
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30. Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model

Author

Matilde Tschon, Elisa Boanini, Maria Sartori, Francesca Salamanna, Silvia Panzavolta, Adriana Bigi, Milena Fini, Tschon, Matilde, Boanini, Elisa, Sartori, Maria, Salamanna, Francesca, Panzavolta, Silvia, Bigi, Adriana, and Fini, Milena

Subjects
Bone Regeneration, Bone Marrow Cells, Zoledronic Acid, Catalysis, Inorganic Chemistry, Tissue Scaffold, Bone Marrow, Osteogenesis, Humans, Physical and Theoretical Chemistry, Molecular Biology, Cells, Cultured, Spectroscopy, Cell Proliferation, Tissue Engineering, Tissue Scaffolds, Organic Chemistry, Cell Differentiation, Mesenchymal Stem Cells, General Medicine, Computer Science Applications, bone fracture, Mesenchymal Stem Cell, in vitro model, human mesenchymal stromal cells, nanohydroxyapatite zoledronate scaffold, bone regeneration, human mesenchymal stromal cell, Bone Marrow Cell, Human
Abstract

Background: Bisphosphonates are widely employed drugs for the treatment of pathologies with high bone resorption, such as osteoporosis, and display a great affinity for calcium ions and apatitic substrates. Here, we aimed to investigate the potentiality of zoledronate functionalized hydroxyapatite nanocrystals (HAZOL) to promote bone regeneration by stimulating adhesion, viability, metabolic activity and osteogenic commitment of human bone marrow derived mesenchymal stromal cells (hMSCs). Methods: we adopted an advanced three-dimensional (3D) in vitro fracture healing model to study porous scaffolds: hMSCs were seeded onto the scaffolds that, after three days, were cut in halves and unseeded scaffolds were placed between the two halves. Scaffold characterization by X-ray diffraction, transmission and scanning electron microscopy analyses and cell morphology, viability, osteogenic differentiation and extracellular matrix deposition were evaluated after 3, 7 and 10 days of culture. Results: Electron microscopy showed a porous and interconnected structure and a uniform cell layer spread onto scaffolds. Scaffolds were able to support cell growth and cells progressively colonized the whole inserts in absence of cytotoxic effects. Osteogenic commitment and gene expression of hMSCs were enhanced with higher expressions of ALPL, COL1A1, BGLAP, RUNX2 and Osterix genes. Conclusion: Although some limitations affect the present study (e.g., the lack of longer experimental times, of mechanical stimulus or pathological microenvironment), the obtained results with the adopted experimental setup suggested that zoledronate functionalized scaffolds (GHAZOL) might sustain not only cell proliferation, but positively influence osteogenic differentiation and activity if employed in bone fracture healing.

Published
2022
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31. Potential Anti-Metastatic Role of the Novel miR-CT3 in Tumor Angiogenesis and Osteosarcoma Invasion

Author

Lavinia Raimondi, Alessia Gallo, Nicola Cuscino, Angela De Luca, Viviana Costa, Valeria Carina, Daniele Bellavia, Matteo Bulati, Riccardo Alessandro, Milena Fini, Pier Giulio Conaldi, Gianluca Giavaresi, Raimondi, Lavinia, Gallo, Alessia, Cuscino, Nicola, De Luca, Angela, Costa, Viviana, Carina, Valeria, Bellavia, Daniele, Bulati, Matteo, Alessandro, Riccardo, Fini, Milena, Conaldi, Pier Giulio, and Giavaresi, Gianluca

Subjects
Epithelial-Mesenchymal Transition, QH301-705.5, MAP Kinase Signaling System, EMT protein, Bone Neoplasms, Article, Catalysis, Cell Line, Inorganic Chemistry, Cell Line, Tumor, Human Umbilical Vein Endothelial Cells, metastasis, Humans, Neoplasm Invasiveness, Biology (General), Physical and Theoretical Chemistry, QD1-999, Molecular Biology, Spectroscopy, Osteosarcoma, Osteoblasts, microRNA, Neovascularization, Pathologic, Organic Chemistry, EMT proteins, tumor angiogenesis, General Medicine, microRNAs, Computer Science Applications, Gene Expression Regulation, Neoplastic, Chemistry, osteosarcoma, metastasi, tumor angiogenesi
Abstract

Osteosarcoma (OS) is the most common primary bone tumor mainly occurring in young adults and derived from primitive bone-forming mesenchyme. OS develops in an intricate tumor microenvironment (TME) where cellular function regulated by microRNAs (miRNAs) may affect communication between OS cells and the surrounding TME. Therefore, miRNAs are considered potential therapeutic targets in cancer and one of the goals of research is to accurately define a specific signature of a miRNAs, which could reflect the phenotype of a particular tumor, such as OS. Through NGS approach, we previously found a specific molecular profile of miRNAs in OS and discovered 8 novel miRNAs. Among these, we deepen our knowledge on the fifth candidate renamed now miR-CT3. MiR-CT3 expression was low in OS cells when compared with human primary osteoblasts and healthy bone. Through TargetScan, VEGF-A was predicted as a potential biological target of miR-CT3 and luciferase assay confirmed it. We showed that enforced expression of miR-CT3 in two OS cell lines, SAOS-2 and MG-63, reduced expression of VEGF-A mRNA and protein, inhibiting tumor angiogenesis. Enforced expression of miR-CT3 also reduced OS cell migration and invasion as confirmed by soft agar colony formation assay. Interestingly, we found that miR-CT3 behaves inducing the activation of p38 MAP kinase pathway and modulating the epithelial-mesenchymal transition (EMT) proteins, in particular reducing Vimentin expression. Overall, our study highlights the novel role of miR-CT3 in regulating tumor angiogenesis and progression in OS cells, linking also to the modulation of EMT proteins.

Published
2022
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