1. Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited Tomato SGR1 Knockout (KO) Line.
- Author
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Kim JY, Kim DH, Kim MS, Jung YJ, and Kang KK
- Subjects
- Ascorbic Acid metabolism, Carotenoids metabolism, Flavonoids metabolism, Fruit genetics, Fruit metabolism, Fruit chemistry, gamma-Aminobutyric Acid metabolism, Gene Editing, Gene Knockout Techniques, Phenols metabolism, Antioxidants metabolism, CRISPR-Cas Systems, Plant Proteins genetics, Plant Proteins metabolism, Solanum lycopersicum genetics, Solanum lycopersicum metabolism
- Abstract
Tomatoes contain many secondary metabolites such as β -carotene, lycopene, phenols, flavonoids, and vitamin C, which are responsible for antioxidant activity. SlSGR1 encodes a STAY-GREEN protein that plays a critical role in the regulation of chlorophyll degradation in tomato leaves and fruits. Therefore, the present study was conducted to evaluate the sgr1 null lines based on their physicochemical characteristics, the content of secondary metabolites, and the γ -Aminobutyric acid (GABA) content. The total soluble solids (TSS), titrated acidity (TA), and brix acid ratio (BAR) of the sgr1 null lines were higher than those of the wild type(WT). Additionally, the sgr1 null lines accumulated higher levels of flavor-inducing ascorbic acid and total carotenoids compared to WT. Also, the total phenolic content, total flavonoids, GABA content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical content of the sgr1 null lines were higher than those of the WT. Therefore, these studies suggest that the knockout of the SGR1 gene by the CRISPR/Cas9 system can improve various functional compounds in tomato fruit, thereby satisfying the antioxidant properties required by consumers.
- Published
- 2024
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