1. The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding.
- Author
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Ehlers, Lisa, Kirchner, Marieluise, Mertins, Philipp, Strehl, Cindy, Buttgereit, Frank, and Gaber, Timo
- Subjects
RECEPTOR antibodies ,MOLECULAR cloning ,IMMUNOGLOBULINS ,TRANSCRIPTION factors ,GLUCOCORTICOID receptors ,MONOCLONAL antibodies - Abstract
Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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