1. Investigating the Anti-Inflammatory, Analgesic, and Chondroprotective Effects of Gynostemma pentaphyllum (Thunb.) Makino in Osteoarthritis: An In Vitro and In Vivo Study.
- Author
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Jo HG, Baek CY, Hwang Y, Baek E, Park C, Song HS, and Lee D
- Subjects
- Animals, Mice, RAW 264.7 Cells, Rats, Male, Cartilage, Articular drug effects, Cartilage, Articular pathology, Cartilage, Articular metabolism, Disease Models, Animal, Rats, Sprague-Dawley, Pain drug therapy, Gynostemma chemistry, Osteoarthritis drug therapy, Osteoarthritis pathology, Osteoarthritis chemically induced, Osteoarthritis metabolism, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents therapeutic use, Plant Extracts pharmacology, Plant Extracts therapeutic use, Analgesics pharmacology, Analgesics therapeutic use
- Abstract
Osteoarthritis (OA) is an age-related disease characterized by inflammation, pain, articular cartilage damage, synovitis, and irreversible disability. Gynostemma pentaphyllum (Thunb.) Makino (GP), a herbal medicine traditionally used in East Asia for its anti-inflammatory properties, was investigated for its potential to modulate OA pathology and symptoms. This study evaluated GP's efficacy in inhibiting pain, functional decline, and cartilage destruction in monosodium iodoacetate-induced OA and acetic acid-induced writhing models. Additionally, the effects of GP on OA-related inflammatory targets were assessed via mRNA and protein expression in rat knee cartilage and lipopolysaccharide-induced RAW 264.7 cells. The GP group demonstrated significant pain relief, functional improvement, and cartilage protection. Notably, GP inhibited key inflammatory mediators, including interleukin (IL)-1β, IL-6, matrix metalloproteinases (MMP)-3 and MMP-13, cyclooxygenase-2, and prostaglandin E receptor 2, surpassing the effects of active controls. These findings suggest that GP is a promising candidate for disease-modifying OA drugs and warrants further comprehensive studies.
- Published
- 2024
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