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Your search keyword '"Friedrich, Oliver"' showing total 7 results
Start Over Author "Friedrich, Oliver" Search Limiters Full Text Journal international journal of molecular sciences Publisher mdpi ag
7 results on '"Friedrich, Oliver"'

2. Partner, Neighbor, Housekeeper and Dimension: 3D versus 2D Glomerular Co-Cultures Reveal Drawbacks of Currently Used Cell Culture Models

Author

Rederer, Anna, primary, Rose, Victoria, additional, Krüger, René, additional, Schmittutz, Linda, additional, Swierzy, Izabela, additional, Fischer, Lena, additional, Thievessen, Ingo, additional, Bauer, Julian, additional, Friedrich, Oliver, additional, Schiffer, Mario, additional, and Müller-Deile, Janina, additional

Published
2023
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4. Myofibrillar Lattice Remodeling Is a Structural Cytoskeletal Predictor of Diaphragm Muscle Weakness in a Fibrotic mdx (mdx Cmah−/−) Model

Author

Ritter, Paul, primary, Nübler, Stefanie, additional, Buttgereit, Andreas, additional, Smith, Lucas R., additional, Mühlberg, Alexander, additional, Bauer, Julian, additional, Michael, Mena, additional, Kreiß, Lucas, additional, Haug, Michael, additional, Barton, Elisabeth, additional, and Friedrich, Oliver, additional

Published
2022
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6. Acidified Nitrite Contributes to the Antitumor Effect of Cold Atmospheric Plasma on Melanoma Cells

Author

Zimmermann, Tom, Gebhardt, Lisa A, Kreiss, Lucas, Schneider, Christin, Arndt, Stephanie, Karrer, Sigrid, Friedrich, Oliver, Fischer, Michael J.M., and Bosserhoff, Anja-Katrin

Subjects
Plasma Gases, Cell Survival, MAP Kinase Signaling System, malignant melanoma, Antineoplastic Agents, Apoptosis, cold atmospheric plasma, nitration, Article, acidified nitrite, lcsh:Chemistry, acidification, lcsh:Biology (General), lcsh:QD1-999, membrane damage, Cell Line, Tumor, Humans, ddc:610, nitrite, Melanoma, lcsh:QH301-705.5, Nitrites, DNA Damage
Abstract

Cold atmospheric plasma (CAP) is partially ionized gas near room temperature with previously reported antitumor effects. Despite extensive research and growing interest in this technology, active components and molecular mechanisms of CAP are not fully understood to date. We used Raman spectroscopy and colorimetric assays to determine elevated nitrite and nitrate levels after treatment with a MiniFlatPlaster CAP device. Previously, we demonstrated CAP-induced acidification. Cellular effects of nitrite and strong extracellular acidification were assessed using live-cell imaging of intracellular Ca2+ levels, cell viability analysis as well as quantification of p21 and DNA damage. We further characterized these observations by analyzing established molecular effects of CAP treatment. A synergistic effect of nitrite and acidification was found, leading to strong cytotoxicity in melanoma cells. Interestingly, protein nitration and membrane damage were absent after treatment with acidified nitrite, thereby challenging their contribution to CAP-induced cytotoxicity. Further, phosphorylation of ERK1/2 was increased after treatment with both acidified nitrite and indirect CAP. This study characterizes the impact of acidified nitrite on melanoma cells and supports the importance of RNS during CAP treatment. Further, it defines and evaluates important molecular mechanisms that are involved in the cancer cell response to CAP.

Published
2021

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