1. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila .
- Author
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Shidlovskii YV, Bylino OV, Shaposhnikov AV, Kachaev ZM, Lebedeva LA, Kolesnik VV, Amendola D, De Simone G, Formicola N, Schedl P, Digilio FA, and Giordano E
- Subjects
- Animals, Animals, Genetically Modified, Drosophila Proteins metabolism, Drosophila melanogaster metabolism, Fluorescent Antibody Technique, Indirect methods, Humans, In Situ Hybridization methods, Models, Genetic, Promoter Regions, Genetic genetics, Protein Subunits genetics, Protein Subunits metabolism, Transcription Factors metabolism, Transcriptional Activation, Chromatin Assembly and Disassembly genetics, Drosophila Proteins genetics, Drosophila melanogaster genetics, Enhancer Elements, Genetic genetics, Transcription Factors genetics, Transcription, Genetic
- Abstract
The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila . These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.
- Published
- 2021
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