1. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila
- Author
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Paul Schedl, Ennio Giordano, Giovanna De Simone, Oleg V. Bylino, Valeria V. Kolesnik, Diego Amendola, Nadia Formicola, Filomena Anna Digilio, A. V. Shaposhnikov, Yulii V. Shidlovskii, Zaur M. Kachaev, Lyubov A. Lebedeva, Shidlovskii, Yulii V, Bylino, Oleg V, Shaposhnikov, Alexander V, Kachaev, Zaur M, Lebedeva, Lyubov A, Kolesnik, Valeria V, Amendola, Diego, De Simone, Giovanna, Formicola, Nadia, Schedl, Paul, Digilio, Filomena Anna, Giordano, Ennio, Shidlovskii, Yulii V., Bylino, Oleg V., Shaposhnikov, Alexander V., Kachaev, Zaur M., Lebedeva, Lyubov A., and Kolesnik, Valeria V.
- Subjects
Transcriptional Activation ,Transcription, Genetic ,Transgene ,Biology ,Article ,Catalysis ,Chromatin remodeling ,chromatin remodeling ,Animals, Genetically Modified ,Inorganic Chemistry ,lcsh:Chemistry ,Chromatin remodeling,Enhancer,PBAP,Promoter,SWI/SNF ,Transcription (biology) ,SWI/SNF ,PBAP ,promoter ,enhancer ,Animals ,Drosophila Proteins ,Humans ,Physical and Theoretical Chemistry ,Fluorescent Antibody Technique, Indirect ,Promoter Regions, Genetic ,Enhancer ,Molecular Biology ,lcsh:QH301-705.5 ,In Situ Hybridization ,Spectroscopy ,Regulation of gene expression ,Models, Genetic ,Organic Chemistry ,Promoter ,General Medicine ,Chromatin Assembly and Disassembly ,Computer Science Applications ,Chromatin ,Cell biology ,Protein Subunits ,Drosophila melanogaster ,Enhancer Elements, Genetic ,lcsh:Biology (General) ,lcsh:QD1-999 ,Transcription Factors - Abstract
The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription.
- Published
- 2021
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