1. Activity of Extracts from Submerged Cultured Mycelium of Winter Mushroom, Flammulina velutipes (Agaricomycetes), on the Immune System In Vitro.
- Author
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Kashina S, Villavicencio LL, Zaina S, Ordaz MB, Sabanero GB, Fujiyoshi VT, and Lopez MS
- Subjects
- Animals, Biological Products chemistry, Biological Products isolation & purification, Candida albicans immunology, Fungal Proteins immunology, Fungal Proteins isolation & purification, Glycoproteins isolation & purification, Humans, Macrophages drug effects, Male, Monocytes drug effects, Mycelium chemistry, Phagocytosis drug effects, Rabbits, Reactive Oxygen Species metabolism, Antibodies metabolism, Biological Products pharmacology, Flammulina chemistry, Glycoproteins immunology, Immune System drug effects
- Abstract
Extracts from submerged cultured mycelium of two strains of Flammulina velutipes, a popular culinary mushroom, were obtained by ultrasound and tested in vitro to determine their activity in innate immunity (monocytes/ macrophages). In addition, polyclonal antibodies against the extracts were produced. Both extracts have similar glycoproteins that contain mannose and glucose but have different glycoproteins with galactoseamine units. Two novel immunogenic glycoproteins with molecular weights of 32 and 25 kDa have been revealed. It is thought that these proteins are produced only by submerged cultured mycelium. Both extracts show immune-enhancing activity based on the significant modification of various parameters such as cytokine production, phagocytosis, and reactive oxygen species production.
- Published
- 2016
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